Phospho-IKB alpha (Ser32) Antibody (YA185)

Cat. No.: HY-P80826: Data Sheet COA
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User Guide for Antibodies
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Phospho-IKB alpha (Ser32) Antibody (YA185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-IKB alpha (Ser32).

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-IKB alpha (Ser32) Antibody (YA185) Featured Recommendations:

1 Publications Citing Use of MCE Phospho-IKB alpha (Ser32) Antibody (YA185)

•J Ethnopharmacol. 2025 Sep 9:120592. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-IKB alpha (Ser32) Antibody (YA185) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-IKB alpha (Ser32).

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 36 kDa;

Observed band size: 36 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P25963

Gene ID

4792 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser32 of Human IKB alpha.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , Hela(lane 3(20ug) and HEK293(lane 4(20ug) using Phospho-IKB alpha Antibody (HY-P80826) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466)

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Induction:Induced in adherent monocytes

Subunit：Interacts with RELA; the interaction requires the nuclear import signal (PubMed:1493333). Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14 (PubMed:9751059). Interacts with NKIRAS1 and NKIRAS2 (PubMed:10657303). Interacts with isoform 1 and isoform 2 of RWDD3; the interaction enhances sumoylation (PubMed:17956732, PubMed:23469069). Interacts with PRMT2 (PubMed:16648481). Interacts with PRKACA in platelets; this interaction is disrupted by thrombin and collagen (PubMed:20356841). Interacts with MEFV (PubMed:18577712). Interacts with DDRGK1; positively regulates NFKBIA phosphorylation and degradation (PubMed:23675531). Interacts with HNRNPA2B1; the interaction may be mediated by the RRM2 domain of HNRNPA2B1, and HNRNPA2B1 may interact simultaneously with FAM76B and either NFKBIA or NFKBIE to form a complex (PubMed:37643469)

RRID

AB_3102722

Database

Entrez Gene: 4792 Human ; 18035 Mouse ; 25493 Rat

SwissProt: P25963 Human ; Q9Z1E3 Mouse ; Q63746 Rat

OMIM: 612132 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-IKB alpha (Ser32) Antibody (YA185) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-IKB alpha (Ser32) Antibody (YA185)

Phospho-IKB alpha (Ser32) Antibody (YA185) Featured Recommendations:

1 Publications Citing Use of MCE Phospho-IKB alpha (Ser32) Antibody (YA185)

Product Detail

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Background

Documentation

Phospho-IKB alpha (Ser32) Antibody (YA185) Related Classifications

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