CAMKK2 Antibody

Cat. No.: HY-P81205: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CAMKK2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CAMKK2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CAMKK2 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CAMKK2 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CAMKK2.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 55 kDa;

Observed band size:65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Sheep Predicted Reactivity: Pig, Cow, Horse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q96RR4

Gene ID

10645 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human CAMKK2: 501-588/588

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
FC FC: Flow Cytometry	1ug:Test

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1) and HCT116 (lane 2) and THP-1 (lane 3) using CAMKK2 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). May play a role in neurite growth. Isoform 3 may promote neurite elongation, while isoform 1 may promoter neurite branching

Subcellular Localization：Nucleus; Cytoplasm; Cell projection, neuron projection

Expression：
Tissue_specificity:Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung

Induction:Up-regulated by PKA pathway

Isoforms & Post-Translational Modification：Q96RR4 has 7 isomers: Q96RR4-1: 64746 Da (predicted); Q96RR4-2: 58899 Da (predicted); Q96RR4-3: 59602 Da (predicted); Q96RR4-4: 59971 Da (predicted); Q96RR4-5: 54827 Da (predicted); Q96RR4-6: 54124 Da (predicted); Q96RR4-7: 61386 Da (predicted).
Autophosphorylated and phosphorylated by PKA. Each isoform may show a different pattern of phosphorylation

Subunit：Interacts with calmodulin

RRID

AB_3103099

Database

Entrez Gene: 10645 Human ; 207565 Mouse ; 83506 Rat

SwissProt: Q96RR4 Human ; Q8C078 Mouse ; O88831 Rat

OMIM: 615002 Human

Synonyms

Calcium/calmodulin dependent protein kinase beta; Calcium/calmodulin dependent protein kinase kinase 2; Calcium/calmodulin dependent protein kinase kinase beta; CaM kinase kinase beta; CaM KK beta; CAMKK 2; CAMKK; CaMKK beta; CAMKK beta protein; CAMKKB.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CAMKK2 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CAMKK2 Antibody

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CAMKK2 Antibody Related Classifications

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