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Bcl-2 Antibody (YA824)(PBS only)

(Synonyms: BCL2; Apoptosis regulator Bcl-2)

Bcl-2 Antibody (YA824) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Bcl-2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Rat, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Bcl-2 Antibody (YA824)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Bcl-2 Antibody (YA824) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Bcl-2.

  • Host Mouse
  • Species Reactivity
    Human, Rat, Mouse
  • Observed Molecular Weight
    Observed band size: 26 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Immunogen

Synthetic peptide corresponding to Human Bcl-2 conjugated to KLH.The exact sequence is proprietary to MCE.

Purification

affinity purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 3T3(lane 2, 20 μg) using AP2M1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
    Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HL-60(lane 4(20ug) using Bcl-2 Antibody (HY-P80565) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Bcl-2 (B-cell lymphoma 2) is a central anti-apoptotic regulator that preserves mitochondrial integrity by preventing mitochondrial outer membrane permeabilization (MOMP), a critical checkpoint controlling cytochrome c release and caspase activation during intrinsic apoptosis[1][2]. Mechanistically, Bcl-2 functions through direct interactions with pro-apoptotic BCL-2 family proteins, including Bax and Bak, thereby suppressing their oligomerization and limiting mitochondrial membrane disruption[3][4]. The protein also integrates signals transmitted by BH3-only proteins, which regulate the balance between cell survival and programmed cell death under physiological and pathological stress conditions[3][5]. Dysregulated Bcl-2 expression contributes to apoptosis resistance in multiple malignancies and supports tumor cell survival by maintaining mitochondrial homeostasis despite cellular stress[6][7]. In disease models, the anti-apoptotic activity of Bcl-2 is closely linked to mitochondrial apoptosis pathways that represent major therapeutic targets in hematologic and solid cancers[6][8]. Compared with related anti-apoptotic isoforms such as Mcl-1 and Bcl-B, Bcl-2 displays broader interactions with both Bax and Bak, whereas Mcl-1 and Bcl-B show preferential regulation of Bak- and Bax-dependent apoptotic pathways, respectively[4]. For experimental applications, BH3-mimetic inhibitors disrupt the interaction between Bcl-2 and pro-apoptotic partners, thereby restoring mitochondrial apoptosis and providing a widely used strategy for mechanistic studies and targeted cancer research[7][8].

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Expression in multiple organizations

  • Isoforms & Post-Translational Modification

    P10415 has 2 isomers: P10415-1: 26266 Da (predicted); P10415-2: 22337 Da (predicted).
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354). Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354). In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354). Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy (PubMed:10567572, PubMed:18570871). Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity);Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity;Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974). Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138). Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630)

  • Subunit

    Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370).

  • SwissProt ID

    P10415

  • Gene ID
    596 [NCBI]
  • Synonyms

    BCL2; Apoptosis regulator Bcl-2

  • Research Field

    Cell Biology

References

[1]. Harris MH, et al. The role of the Bcl-2 family in the regulation of outer mitochondrial membrane permeability. Cell Death Differ. 2000;7:1182-1191.

[2]. Breckenridge DG, et al. Regulation of mitochondrial membrane permeabilization by BCL-2 family proteins and caspases. Curr Opin Cell Biol. 2004;16(6):647-652.

[3]. Kale J, et al. BCL-2 family proteins: changing partners in the dance towards death. Cell Death Differ. 2018;25:65-80.

[4]. Zhai D, et al. Differential regulation of Bax and Bak by anti-apoptotic Bcl-2 family proteins Bcl-B and Mcl-1. J Biol Chem. 2008 Apr 11;283(15):9580-6. [Content Brief]

[5]. Lomonosova E, et al. BH3-only proteins in apoptosis and beyond: an overview. Oncogene. 2008 Dec;27 Suppl 1(Suppl 1):S2-19. [Content Brief]

[6]. Danial NN. BCL-2 family proteins: critical checkpoints of apoptotic cell death. Clin Cancer Res. 2007;13(24):7254-7263.

[7]. Akl H, et al. A dual role for the anti-apoptotic Bcl-2 protein in cancer: Mitochondria versus endoplasmic reticulum. Biochim Biophys Acta. 2014;1843(10):2240-2252.

[8]. Kang MH, et al. Bcl-2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy. Clin Cancer Res. 2009 Feb 15;15(4):1126-32. [Content Brief]

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Bcl-2 Antibody (YA824)(PBS only) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 3T3(lane 2, 20 μg) using AP2M1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HL-60(lane 4(20ug) using Bcl-2 Antibody (HY-P80565) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Bcl-2 Antibody (YA824)(PBS only) Product Image
    1/16
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Bcl-2 Antibody (YA824)(PBS only) Product Image
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 3T3(lane 2, 20 μg) using AP2M1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HL-60(lane 4(20ug) using Bcl-2 Antibody (HY-P80565) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Experimental Validation Results for Bcl-2 Antibody (YA824)(PBS only)
  • Bcl-2 Antibody (YA824)(PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 3T3(lane 2, 20 μg) using AP2M1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Bcl-2 Antibody (YA824)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HL-60(lane 4(20ug) using Bcl-2 Antibody (HY-P80565) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Bcl-2 Antibody (YA824)(PBS only) Product Image WB
  • Bcl-2 Antibody (YA824)(PBS only) Product Image WB