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STAT5 Antibody(YA054) (PBS only)

(Synonyms: MGF; STAT5)
1 Cited Publications
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Based on 1 publication(s) in Google Scholar

STAT5 Antibody(YA054) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • STAT5 Antibody(YA054) (PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:200-1:2000 1:100-1:200 1:50-1:100 1:50-1:100

Product Details

Description

STAT5 Antibody(YA054) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to STAT5.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Immunogen

Synthetic peptide corresponding to Mouse STAT5A aa600-786.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718730

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, pH 7.4.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
    Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
    Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    STAT5 comprises STAT5A and STAT5B, transcription factors that regulate hematopoietic cell proliferation, differentiation, and apoptosis[1]. Mechanistically, STAT5 tyrosine phosphorylation in peripheral T cells is driven by cytokine receptor signaling rather than the T-cell receptor complex[2]. This cytokine-linked pathway supports immune regulation, lymphocyte development, and cell survival programs[3]. In disease contexts, STAT5B deficiency is associated with growth failure, immunodeficiency, and severe pulmonary disease[4]. Compared with STAT5A, STAT5B shows dominant activity in effector and regulatory T-cell responses, with paralog dose explaining much functional specificity[5]. In BCR/ABL leukemia models, STAT5B, but not STAT5A, serves as the major STAT5 isoform driving leukemogenesis[6]. For experimental applications, AC-4-130 directly binds STAT5 and disrupts activation, dimerization, nuclear translocation, and STAT5-dependent transcription in AML models[7]. Pimozide also decreases survival of chronic myelogenous leukemia cells resistant to kinase inhibitors[8].

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:In adolescent females, it is present in almost all tissues except the brain and muscles. In lactating women, it is abundant in mammary gland tissue and other secretory organs (such as salivary glands and seminal vesicles) .

  • Subunit

    Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 and SOCS7 (By similarity). Binds NR3C1 (PubMed:9528750). Interacts with ERBB4 (PubMed:10508857, PubMed:16837552). Interacts with EBF4 (By similarity). Interacts with CD69 (PubMed:20696842)

  • SwissProt ID

    P42230

  • Gene ID
    20850 [NCBI]
  • Synonyms

    MGF; STAT5

  • Research Field

    Signal Transduction

[1]. Nosaka T, et al. STAT5 as a molecular regulator of proliferation, differentiation and apoptosis in hematopoietic cells. EMBO J. 1999 Sep 1;18(17):4754-65. [Content Brief]

[2]. Moriggl R, et al. Stat5 activation is uniquely associated with cytokine signaling in peripheral T cells. Immunity. 1999 Aug;11(2):225-30. [Content Brief]

[3]. Smith MR, et al. STAT5b: A master regulator of key biological pathways. Front Immunol. 2023 Jan 23;13:1025373. [Content Brief]

[4]. Nadeau K, et al. STAT5b deficiency: an unsuspected cause of growth failure, immunodeficiency, and severe pulmonary disease. J Pediatr. 2011 May;158(5):701-8. [Content Brief]

[5]. Villarino A, et al. Signal transducer and activator of transcription 5 (STAT5) paralog dose governs T cell effector and regulatory functions. Elife. 2016 Mar 21;5:e08384. [Content Brief]

[6]. Kollmann S, et al. Twins with different personalities: STAT5B-but not STAT5A-has a key role in BCR/ABL-induced leukemia. Leukemia. 2019 Jul;33(7):1583-1597. [Content Brief]

[7]. Wingelhofer B, et al. Pharmacologic inhibition of STAT5 in acute myeloid leukemia. Leukemia. 2018 May;32(5):1135-1146. [Content Brief]

[8]. Nelson EA, et al. The STAT5 inhibitor pimozide decreases survival of chronic myelogenous leukemia cells resistant to kinase inhibitors. Blood. 2011 Mar 24;117(12):3421-9. [Content Brief]

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STAT5 Antibody(YA054) (PBS only) Related Classifications

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Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • STAT5 Antibody(YA054) (PBS only) Product Image
    1/16
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • STAT5 Antibody(YA054) (PBS only) Product Image
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • Experimental Validation Results for STAT5 Antibody(YA054) (PBS only)
  • STAT5 Antibody(YA054) (PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from PC-12 (lane 1, 20 μg), C6 (lane 2, 20 μg), RAW264.7 (lane 3, 20 μg), 3T3 (lane 4, 20 μg), HeLa (lane 5, 20 μg), and A549 (lane 6, 20 μg) using STAT5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • STAT5 Antibody(YA054) (PBS only) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from 293T (lane 1) and K562 (lane 2) and HepG2 (lane 3) using STAT5 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • STAT5 Antibody(YA054) (PBS only) Product Image WB
  • STAT5 Antibody(YA054) (PBS only) Product Image WB