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TRIM29 Antibody (YA2552)

(Synonyms: ATDC; TRIM29)
Customer Review

Based on 1 Customer Validation

TRIM29 Antibody (YA2552) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM29.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRIM29 Antibody (YA2552)
  • TRIM29 Antibody (YA2552) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

TRIM29 Antibody (YA2552) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIM29.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 66 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human TRIM29 aa44-100/588.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104901

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIM29 Antibody (YA2552)
    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Mouse thymus (lane 3, 15 μg) using TRIM29 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    TRIM29 plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation

  • Subcellular Localization

    Cytoplasm; Lysosome

  • Expression


    Tissue_specificity:Expressed in placenta, prostate and thymus

  • Isoforms & Post-Translational Modification

    Q14134 has 2 isomers: Q14134-1: 65835 Da (predicted); Q14134-2: 63844 Da (predicted).
    Constitutively phosphorylated by PKC on serine/threonine in A431 cells

  • Subunit

    Interacts with VIM and HINT1 (PubMed:7644499). Interacts with IKBKG/NEMO (PubMed:27695001). Interacts with STING1 (PubMed:29038422)

  • SwissProt ID

    Q14134

  • Gene ID
    23650 [NCBI]
  • Synonyms

    ATDC; TRIM29

  • Research Field

    Epigenetics and Nuclear Signaling

TRIM29 Antibody (YA2552) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Mouse thymus (lane 3, 15 μg) using TRIM29 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRIM29 Antibody (YA2552) Product Image
    1/16
  • Experimental Validation Results for TRIM29 Antibody (YA2552)
  • TRIM29 Antibody (YA2552) Product Image
  • Experimental Validation Results for TRIM29 Antibody (YA2552)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Mouse thymus (lane 3, 15 μg) using TRIM29 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TRIM29 Antibody (YA2552)
  • TRIM29 Antibody (YA2552) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from HeLa (lane 1, 15 μg), Daudi (lane 2, 15 μg), and Mouse thymus (lane 3, 15 μg) using TRIM29 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TRIM29 Antibody (YA2552) Product Image WB