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Ezrin Antibody (YA767)

(Synonyms: EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81)
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Based on 1 Customer Validation

Ezrin Antibody (YA767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Ezrin Antibody (YA767)
  • Ezrin Antibody (YA767) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Ezrin Antibody (YA767) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ezrin.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 81 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Ezrin.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102946

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ezrin Antibody (YA767)
    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Ezrin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis

  • Subcellular Localization

    Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side; Cytoplasm, cell cortex; Cytoplasm, cytoskeleton; Cell projection, microvillus

  • Expression


    Tissue_specificity:It is expressed in the cerebral cortex, basal ganglia, hippocampus, pituitary gland, and optic nerve. Expression is weaker in the brainstem and diencephalon. Expression is stronger in the frontal gray matter than in white matter (protein level) . It is a component of the microvilli of intestinal epithelial cells. It is preferentially expressed in astrocytes of the hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Expression was not detected in neurons in most of the tissues studied.

  • Subunit

    Monomer. Homodimer (PubMed:27364155). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, NHERF1 and TMEM8B (PubMed:12651155, PubMed:15498789, PubMed:17616675, PubMed:17881735, PubMed:19555689, PubMed:9314537).

  • SwissProt ID

    P15311

  • Gene ID
    7430 [NCBI]
  • Synonyms

    EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

  • Research Field

    Cell Biology

Ezrin Antibody (YA767) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Ezrin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Ezrin Antibody (YA767) Product Image
    1/16
  • Experimental Validation Results for Ezrin Antibody (YA767)
  • Ezrin Antibody (YA767) Product Image
  • Experimental Validation Results for Ezrin Antibody (YA767)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Ezrin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Ezrin Antibody (YA767)
  • Ezrin Antibody (YA767) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Ezrin Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Ezrin Antibody (YA767) Product Image WB