ELK1 Antibody (YA2758)(PBS only)
(Synonyms: ETS domain-containing protein Elk-1)ELK1 Antibody (YA2758) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELK1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP, ChIP
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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ChIP
ChIP: Chromatin Immunoprecipitation
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 | 1:20 |
Product Details
ELK1 Antibody (YA2758) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ELK1.
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Host Rabbit
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 62 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 45 kDa
A synthetic peptide of human ELK1 aa408-428.
Affinity Purified
Non-conjugated
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ELK1 transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation[1][2][3].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:lungs and testes -
Isoforms & Post-Translational Modification
P19419 has 2 isomers: P19419-1: 44888 Da (predicted); P19419-2: 11217 Da (predicted).
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention;On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity). Phosphorylated by PRP4K at Thr-417; phosphorylation activation ELK1 transcriptional activity (PubMed:10799319) -
Subunit
Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity (PubMed:15920481). Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9 (PubMed:17296730). Interacts with POU1F1 (PubMed:26612202)
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SwissProt ID
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Synonyms
ETS domain-containing protein Elk-1
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Research Field
Signal Transduction
Documentation
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