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IL-10 Antibody (YA4644)

(Synonyms: Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. 白介素10)
Customer Review

Based on 1 Customer Validation

IL-10 Antibody (YA4644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-10.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • IL-10 Antibody (YA4644) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000 1:500 1:1000

Product Details

Description

IL-10 Antibody (YA4644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-10.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Synthetic peptide within Human IL-10 aa 1-100.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719013

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IL-10 Antibody (YA4644)
    Western blot analysis of extracts from Rat spleen tissue (lane2, 20μg), THP-1 (lane3, 20μg) and HL-60 (lane4, 20μg) using IL-10 Antibody (HY-P84952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-10 Antibody (YA4644)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-10 Antibody (YA4644)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Interleukin-10 (IL-10) functions as a critical anti-inflammatory cytokine that modulates innate and adaptive immune responses[1][2]. Mechanistically, IL-10 activates the JAK-STAT signaling pathway, particularly STAT3, to suppress proinflammatory cytokine production and promote immunoregulatory gene expression[3][1]. In experimental models of bacterial sepsis, IL-10 partially restores mitochondrial function and oxidative phosphorylation in monocytes, counteracting pathogen-induced metabolic inhibition[1]. T cell-derived IL-10 production is regulated by metabolic pathways, including aryl hydrocarbon receptor (Ahr) and Bhlhe40, which control cytokine output and mucosal immune homeostasis in intestinal inflammation models[2]. Compared with related cytokines, IL-10 displays distinct anti-inflammatory potency and feedback regulation not shared by IL-6 or TNF-α[3][1]. Agonists and inhibitors targeting upstream metabolic or signaling modulators, such as dichloroacetate (DCA), can selectively enhance IL-10 production in Th1 cells, demonstrating translational potential for inflammatory bowel disease research[2]. Furthermore, IL-10 isoform-specific signaling contributes to differential regulation of oxidative phosphorylation genes, offering a mechanistic basis for isoform-focused experimental applications[1]. Collectively, these findings highlight IL-10 as a central regulator of immune homeostasis, with mechanistic specificity distinct from related cytokines and amenable to pharmacological modulation in experimental and disease-relevant contexts[3][1][2].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is generated from a variety of cell lines, including T cells, macrophages, mast cells, and other cell types.
    Positive sample: THP-1 cell lysate, THP-1 treated with 800nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate,

  • Subunit

    Homodimer (PubMed:11485736, PubMed:15837194). Interacts with IL10RA and IL10RB (PubMed:16982608)

  • SwissProt ID

    P22301

  • Gene ID
    3586 [NCBI]
  • Synonyms

    Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. 白介素10

References

[1]. Cheon H, et al. Prostaglandin E2 augments IL-10 signaling and function. J Immunol. 2006 Jul 15;177(2):1092-100. [Content Brief]

[2]. Gopinathan U, et al. Transcriptomic data from two primary cell models stimulating human monocytes suggest inhibition of oxidative phosphorylation and mitochondrial function by N. meningitidis which is partially up-regulated by IL-10. BMC Immunol. 2017 Oct 27;18(1):46. [Content Brief]

[3]. Liu H, et al. Metabolic regulation of T cell production of IL-10 and IL-22 protects against intestinal inflammation. Precis Clin Med. 2025 Oct 24;8(4):pbaf025. [Content Brief]

IL-10 Antibody (YA4644) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Rat spleen tissue (lane2, 20μg), THP-1 (lane3, 20μg) and HL-60 (lane4, 20μg) using IL-10 Antibody (HY-P84952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-10 Antibody (YA4644) Product Image
    1/16
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • IL-10 Antibody (YA4644) Product Image
  • Experimental Validation Results for IL-10 Antibody (YA4644)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Rat spleen tissue (lane2, 20μg), THP-1 (lane3, 20μg) and HL-60 (lane4, 20μg) using IL-10 Antibody (HY-P84952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IL-10 Antibody (YA4644)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-10 Antibody (YA4644)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • Experimental Validation Results for IL-10 Antibody (YA4644)
  • IL-10 Antibody (YA4644) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Rat spleen tissue (lane2, 20μg), THP-1 (lane3, 20μg) and HL-60 (lane4, 20μg) using IL-10 Antibody (HY-P84952). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • IL-10 Antibody (YA4644) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-10 Antibody (YA4644) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using IL-10 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IL-10 Antibody (YA4644) Product Image WB
  • IL-10 Antibody (YA4644) Product Image IHC-P
  • IL-10 Antibody (YA4644) Product Image IHC-P