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GABRB1 Antibody (YA10005)

(Synonyms: Gamma-aminobutyric acid receptor subunit beta-1, GABA(A) receptor subunit beta-1, GABAAR subunit beta-1, GABRB1)

GABRB1 Antibody (YA10005) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to GABRB1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GABRB1 Antibody (YA10005)
  • GABRB1 Antibody (YA10005) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-2000 1:50-200

Product Details

Description

GABRB1 Antibody (YA10005) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to GABRB1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Immunogen

Synthetic peptide corresponding to human GABRB1 protein.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GABRB1 Antibody (YA10005)
    Western blot analysis was performed on protein extracts (40 μg) from Rat brain (lane 2) and Mouse brain (lane 3) using GABRB1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    GABRB1 is a Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain. GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain one or two GABA active binding sites located at the alpha and beta subunit interfaces, depending on subunit composition (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient. Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission. Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (By similarity)[1][2][3].

  • Subcellular Localization

    Postsynaptic cell membrane; Cell membrane

  • Isoforms & Post-Translational Modification

    GABRB1 has 2 isoforms, P18505-1: amino acid length is 474, molecular weight is 54235 Da (predicted); P18505-2: amino acid length is 87, molecular weight is 10151 Da (predicted).

  • Subunit

    Heteropentamer, formed by a combination of alpha (GABRA1-6), beta (GABRB1-3), gamma (GABRG1-3), delta (GABRD), epsilon (GABRE), rho (GABRR1-3), pi (GABRP) and theta (GABRQ) chains, each subunit exhibiting distinct physiological and pharmacological properties.

  • SwissProt ID

    P18505

  • Gene ID
    2560 [NCBI]
  • Synonyms

    Gamma-aminobutyric acid receptor subunit beta-1, GABA(A) receptor subunit beta-1, GABAAR subunit beta-1, GABRB1

References

[1]. Bonnert TP, et al. theta, a novel gamma-aminobutyric acid type A receptor subunit. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9891-6. [Content Brief]

[2]. Ranna M, et al. Impact of epsilon and theta subunits on pharmacological properties of alpha3beta1 GABAA receptors expressed in Xenopus oocytes. BMC Pharmacol. 2006 Jan 13;6:1. [Content Brief]

[3]. Janve VS, et al. Epileptic encephalopathy de novo GABRB mutations impair γ-aminobutyric acid type A receptor function. Ann Neurol. 2016 May;79(5):806-825. [Content Brief]

GABRB1 Antibody (YA10005) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from Rat brain (lane 2) and Mouse brain (lane 3) using GABRB1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • GABRB1 Antibody (YA10005) Product Image
    1/16
  • Experimental Validation Results for GABRB1 Antibody (YA10005)
  • GABRB1 Antibody (YA10005) Product Image
  • Experimental Validation Results for GABRB1 Antibody (YA10005)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (40 μg) from Rat brain (lane 2) and Mouse brain (lane 3) using GABRB1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for GABRB1 Antibody (YA10005)
  • GABRB1 Antibody (YA10005) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (40 μg) from Rat brain (lane 2) and Mouse brain (lane 3) using GABRB1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • GABRB1 Antibody (YA10005) Product Image WB