Topoisomerase I Antibody (YA2237)(PBS only)

Cat. No.: HY-P82492A: Data Sheet User Guide for Antibodies
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Topoisomerase I Antibody (YA2237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase I.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Topoisomerase I Antibody (YA2237) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Topoisomerase I.

Host

Rabbit

분자량

Predicted band size: 91 kDa;

Observed band size: 91 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11387

Gene ID

7150 [NCBI]

Immunogen

A synthetic peptide of human Topoisomerase I aa2-15.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter

Subcellular Localization：Nucleus, nucleolus; Nucleus, nucleoplasm

Expression：
Tissue_specificity:Endothelial cells

Subunit：Monomer. Interacts with ERCC6 (PubMed:26030138). Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)

Research Field

Epigenetics and Nuclear Signaling

Synonyms

TOP1; DNA topoisomerase 1; DNA topoisomerase I

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/a549-cell.html [Content Brief]