ST2 Antibody (YA6958)

Cat. No.: HY-P87275: Data Sheet COA
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ST2 Antibody (YA6958) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ST2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

ST2 Antibody (YA6958) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ST2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 63.4 kDa;

Observed band size: 80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q01638

Immunogen

Recombinant protein within human ST2 aa 19-328 (Extracellular).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
FC FC: Flow Cytometry	1:1000

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Human kidney (lane 1(40μg)) 、Human lung (lane 2(40μg)) and Human lung cancer (lane 3(40μg)) using ST2 Antibody (HY-P87275) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP (PubMed:35238669). Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function (Probable) (PubMed:16286016). Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity)|Inhibits IL-33 signaling

Subcellular Localization：Cell membrane,Secreted,Cell membrane

Expression：
Tissue_Specificity: Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Expressed on T-cells in fibrotic liver; at protein level. Overexpressed in fibrotic and cirrhotic liver

Isoforms & Post-Translational Modification：Q01638 has four isomers: Q01638-1: 63358 Da (predicted); Q01638-2: 36993 Da (predicted); Q01638-3: 29951 Da (predicted); Q01638-4: 23723 Da (predicted).
Ubiquitinated at Lys-321 in a FBXL19-mediated manner; leading to proteasomal degradation

Subunit：Interacts with MYD88, IRAK1, IRAK4, and TRAF6

RRID

AB_3719303

Synonyms

DER-4 antibody; DER4 antibody; FIT 1 antibody; Growth stimulation expressed antibody; homolog of mouse growth stimulation-expressed antibody; Il1rl1 antibody; IL33R antibody; ILRL1_HUMAN antibody; Interleukin 1 receptor like 1 antibody; interleukin 1 receptor related protein antibody

Documentation

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/huh-7-cell.html [Content Brief]