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GLB1/Beta-galactosidase Antibody (YA2816)

(Synonyms: GLB1; ELNR1; Beta-galactosidase; Acid beta-galactosidase; Lactase; Elastin receptor 1)
Customer Review

Based on 1 Customer Validation

GLB1/Beta-galactosidase Antibody (YA2816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GLB1/Beta-galactosidase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

GLB1/Beta-galactosidase Antibody (YA2816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GLB1/Beta-galactosidase.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 95 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 76 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human GLB1 aa621-677/677.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105025

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
    Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and K562(lane 4(20ug) using GLB1 Antibody (HY-P83071) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
    Flow cytometric analysis of 1X10^6 A549 cells labeling GLB1 Antibody (YA2816) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    GLB1/Beta-galactosidase cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans; Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers

  • Subcellular Localization

    Lysosome; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:Protein levels were detected in the placenta (PubMed: 8383699) . It was also detected in fibroblasts and testes (PubMed: 2511208) .

  • Isoforms & Post-Translational Modification

    P16278 has 3 isomers: P16278-1: 76075 Da (predicted); P16278-2: 60536 Da (predicted); P16278-3: 72751 Da (predicted).

  • Subunit

    Homodimer (PubMed:22128166). May form higher multimers (Probable)

  • SwissProt ID

    P16278

  • Gene ID
    2720 [NCBI]
  • Synonyms

    GLB1; ELNR1; Beta-galactosidase; Acid beta-galactosidase; Lactase; Elastin receptor 1

  • Research Field

    Tags & Cell Markers

GLB1/Beta-galactosidase Antibody (YA2816) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and K562(lane 4(20ug) using GLB1 Antibody (HY-P83071) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling GLB1 Antibody (YA2816) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image
    1/16
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and K562(lane 4(20ug) using GLB1 Antibody (HY-P83071) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 A549 cells labeling GLB1 Antibody (YA2816) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • Experimental Validation Results for GLB1/Beta-galactosidase Antibody (YA2816)
  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and K562(lane 4(20ug) using GLB1 Antibody (HY-P83071) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 A549 cells labeling GLB1 Antibody (YA2816) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image WB
  • GLB1/Beta-galactosidase Antibody (YA2816) Product Image FC