FADD Antibody (YA2793)(PBS only)

Cat. No.: HY-P83048A: Data Sheet User Guide for Antibodies
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FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

Host

Rabbit

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 28 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q61160

Gene ID

8772 [NCBI]

Immunogen

A synthetic peptide of mouse FADD

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling

Subcellular Localization：Cytoplasm

Subunit：Can self-associate (By similarity). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (PubMed:34471287). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (By similarity). Interacts (via death domain) with FAS (via death domain) (By similarity). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280). Interacts with CFLAR, PEA15 and MBD4 (By similarity). When phosphorylated, part of a complex containing HIPK3 and FAS (By similarity). May interact with MAVS/IPS1 (By similarity). Interacts with MOCV v-CFLAR protein and PIDD1 (By similarity). Interacts with RIPK1 and TRADD (PubMed:28842570, PubMed:29440439, PubMed:30185824, PubMed:31519887). Interacts with stimulated TNFRSF10B (By similarity). Interacts with DDX24 (By similarity)

Research Field

Cell Biology

Synonyms

Mort1/FADD

Documentation

User Guide for Antibodies (1077 KB)