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FADD Antibody (YA2793)(PBS only)

(Synonyms: Mort1/FADD)

FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for FADD Antibody (YA2793)(PBS only)
  • FADD Antibody (YA2793)(PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

FADD Antibody (YA2793) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to FADD.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 23 kDa
Immunogen

A synthetic peptide of mouse FADD

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for FADD Antibody (YA2793)(PBS only)
    Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    FADD is an Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling

  • Subcellular Localization

    Cytoplasm

  • Subunit

    Can self-associate (By similarity). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (PubMed:34471287). Component of the death-induced signaling complex (DISC) composed of cell surface receptor FAS/CD95 or TNFRSF1A, adapter protein FADD and the CASP8 protease; recruitment of CASP8 to the complex is required for processing of CASP8 into the p18 and p10 subunits (By similarity). Interacts (via death domain) with FAS (via death domain) (By similarity). Interacts directly (via DED domain) with NOL3 (via CARD domain); inhibits death-inducing signaling complex (DISC) assembly by inhibiting the increase in FAS-FADD binding induced by FAS activation (PubMed:15383280). Interacts with CFLAR, PEA15 and MBD4 (By similarity). When phosphorylated, part of a complex containing HIPK3 and FAS (By similarity). May interact with MAVS/IPS1 (By similarity). Interacts with MOCV v-CFLAR protein and PIDD1 (By similarity). Interacts with RIPK1 and TRADD (PubMed:28842570, PubMed:29440439, PubMed:30185824, PubMed:31519887). Interacts with stimulated TNFRSF10B (By similarity). Interacts with DDX24 (By similarity)

  • SwissProt ID

    Q61160

  • Gene ID
    8772 [NCBI]
  • Synonyms

    Mort1/FADD

  • Research Field

    Cell Biology

FADD Antibody (YA2793)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FADD Antibody (YA2793)(PBS only) Product Image
    1/16
  • Experimental Validation Results for FADD Antibody (YA2793)(PBS only)
  • FADD Antibody (YA2793)(PBS only) Product Image
  • Experimental Validation Results for FADD Antibody (YA2793)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for FADD Antibody (YA2793)(PBS only)
  • FADD Antibody (YA2793)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 3T3 (lane 1, 20 μg), 3T3+CA (lane 2, 20 μg), SH-SY5Y (lane 3, 20 μg), and SH-SY5Y+CA (lane 4, 20 μg) using FADD Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • FADD Antibody (YA2793)(PBS only) Product Image WB