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Phospho-CDC6 (Ser54) Antibody (YA1017)

(Synonyms: CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6)
Customer Review

Based on 1 Customer Validation

Phospho-CDC6 (Ser54) Antibody (YA1017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-CDC6 (Ser54).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Phospho-CDC6 (Ser54) Antibody (YA1017) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-CDC6 (Ser54).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 63 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Species Reactivity Database

Entrez Gene: 990 Human

SwissProt: Q99741 Human

Immunogen

A synthesized peptide derived from human Phospho-Cdc6 (S54)

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3103388

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
    Immunocytochemistry analysis of A549 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
    Immunocytochemistry analysis of C6 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CDC6 is involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Subunit

    Interacts with PCNA, ORC1, cyclin-CDK (PubMed:9566895). Interacts with HUWE1 (PubMed:17567951). Interacts with ANKRD17 (PubMed:23711367). Interacts with GRWD1; origin binding of GRWD1 is dependent on CDC6 (PubMed:25990725). Interacts with CDT1; are mutually dependent on one another for loading MCM complexes onto chromatin (PubMed:14672932). Interacts with TTC4 (PubMed:18320024). Interacts (via Cy motif) with CCNF; the interaction takes place during G2 and M phase (PubMed:26818844). Interacts with CDH1 (PubMed:26818844)

  • SwissProt ID

    Q99741

  • Gene ID
    990 [NCBI]
  • Synonyms

    CDC6; CDC18L; Cell division control protein 6 homolog; CDC6-related protein; Cdc18-related protein; HsCdc18; p62(cdc6); HsCDC6

  • Research Field

    Epigenetics and Nuclear Signaling

Phospho-CDC6 (Ser54) Antibody (YA1017) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image
    1/16
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Experimental Validation Results for Phospho-CDC6 (Ser54) Antibody (YA1017)
  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Phospho-CDC6 (Ser54)Antibody (HY-P81295A) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-CDC6 (Ser54) Antibody (HY-P81295A) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ Overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image ICC
  • Phospho-CDC6 (Ser54) Antibody (YA1017) Product Image ICC