PMCA1 Antibody (YA1676)(PBS only)

Cat. No.: HY-P81931A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

PMCA1 Antibody (YA1676) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMCA1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PMCA1 Antibody (YA1676) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PMCA1.

Host

Rabbit

Molecular Weight

Predicted band size: 135 kDa;

Observed band size: 130-250 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P20020

Gene ID

490 [NCBI]

Immunogen

A synthetic peptide of human PMCA1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis (PubMed:35358416). Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511). May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Synapse; Presynaptic cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Isoform B: Ubiquitously expressed. Isoform C: Found in brain cortex, skeletal muscle and heart muscle. Isoform D: Has only been found in fetal skeletal muscle. Isoform K: Found in small intestine and liver. Abundantly expressed in the endometrial epithelial cells and glandular epithelial cells in early-proliferative phase and early-secretory phases (PubMed:21400627)

Induction:Up-regulated at the proliferative phase of the mentrual cycle. Up-regulated by estrogen

Isoforms & Post-Translational Modification：P20020 has 6 isomers: P20020-3: 134685 Da (predicted); P20020-1: 138755 Da (predicted); P20020-2: 129516 Da (predicted); P20020-4: 137804 Da (predicted); P20020-5: 129152 Da (predicted); P20020-6: 130617 Da (predicted).

Subunit：Monomer (PubMed:1332771). Dimer (PubMed:1332771). Oligomer (PubMed:1332771). Calmodulin binding (PubMed:1332771). Interacts with PDZD11 (PubMed:12763866).

Research Field

Signal Transduction

Synonyms

ATP2B1; PMCA1

Documentation

User Guide for Antibodies (1077 KB)