OGT Antibody (YA7208)
(Synonyms: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT)Based on 1 Customer Validation
OGT Antibody (YA7208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to OGT.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, IF-Tissue, FC, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 0.01M tris buffered saline(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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FC
FC: Flow Cytometry
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 | 1:100-200 | 1:100-200 |
Product Details
OGT Antibody (YA7208) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to OGT.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat Predicted Reactivity: Mouse,Rat
Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
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Observed Molecular WeightObserved band size: 110 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 117 kDa
A synthesized peptide derived from human OGT: 1000-1046.
Endogenous
affinity purified by Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M tris buffered saline(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
OGT (O-GlcNAc transferase) catalyzes the addition of the O-GlcNAc post-translational modification to proteins. O-GlcNAcylation is a dynamic form of protein glycosylation which involves the addition of β-d-N-acetylglucosamine (GlcNAc) via an O-linkage to serine or threonine residues of nuclear, cytoplasmic, mitochondrial and transmembrane proteins.
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Subcellular Localization
Nucleus,Cytoplasm,Mitochondrion,Membrane,Cytoplasm,Nucleus,Cell membrane,Mitochondrion membrane,Cell projection,Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver
Induction: Induction of the nucleocytoplasmic OGT (ncOGT) isoform in the liver on glucose deprivation is mediated by the decreased hexosamine biosynthesis pathway (HBP) flux -
Isoforms & Post-Translational Modification
O15294 has four isomers: O15294-1: 116925 Da (predicted); O15294-2: 103012 Da (predicted); O15294-3: 115706 Da (predicted); O15294-4: 74536 Da (predicted).
Ubiquitinated by the SCF(FBXO31) complex, leading to its proteasomal degradation丨Phosphorylation on Ser-3 or Ser-4 by GSK3-beta positively regulates its activity (By similarity)丨Glycosylated via autocatalysis; O-GlcNAcylation at Ser-399 promotes nuclear localization丨Glycosylated via autocatalysis; does not affect the enzyme activity but regulates substrate selectivity -
Subunit
Monomer; may exist in different oligomerization states in cells (PubMed:21240259, PubMed:27713473)
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SwissProt ID
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Synonyms
UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT
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