Acetyl Coenzyme A Carboxylase Antibody (YA644)(PBS only)

Cat. No.: HY-P80528A: Data Sheet User Guide for Antibodies
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Acetyl Coenzyme A Carboxylase Antibody (YA644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Acetyl Coenzyme A Carboxylase Antibody (YA644) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Acetyl Coenzyme A Carboxylase.

Host

Rabbit

Molecular Weight

Predicted band size: 277 kDa;

Observed band size: 277 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O00763

Gene ID

32 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Acetyl Coenzyme A Carboxylase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	affinity purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed:16854592, PubMed:19236960, PubMed:19900410, PubMed:20457939, PubMed:20952656, PubMed:26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed:19236960, PubMed:20457939, PubMed:20952656, PubMed:26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity)

Subcellular Localization：Mitochondrion

Expression：
Tissue_specificity:This gene is widely expressed in the heart, skeletal muscle, liver, adipose tissue, mammary gland, adrenal gland, and colon, with the highest expression levels found in these areas (PubMed: 9099716) . Isomer 3 is expressed in skeletal muscle, adipose tissue, and liver (protein level) (PubMed: 19190759) . Isomer 3 is present in higher amounts in adipose tissue and in lower amounts in the heart, liver, skeletal muscle, and testes (PubMed: 19190759) .

Isoforms & Post-Translational Modification：O00763 has 3 isomers: O00763-1: 276541 Da (predicted); O00763-2: 268166 Da (predicted); O00763-3: 255093 Da (predicted).
The biotin cofactor is covalently attached to the central biotinyl-binding domain and is required for the catalytic activity;Phosphorylation at Ser-222 by AMPK inactivates the enzyme (PubMed:12488245). Required for the maintenance of skeletal muscle lipid and glucose homeostasis (By similarity)

Subunit：Monomer, homodimer, and homotetramer (PubMed:18772397, PubMed:20952656). Forms filamentous polymers (PubMed:19900410, PubMed:20457939, PubMed:20952656). Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity in a citrate-dependent manner (PubMed:20457939, PubMed:20952656)

Research Field

Cell Biology

Synonyms

ACC; ACAC; ACC1; ACCA; ACACAD

Documentation

User Guide for Antibodies (1077 KB)