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NCOA4 Antibody (YA4630)

(Synonyms: 70 kDa androgen receptor activator antibody; 70 kDa androgen receptor coactivator antibody; 70 kDa AR activator antibody; 70 kDa AR-activator antibody; Androgen receptor coactivator 70 kD antibody; Androgen receptor coactivator 70 kDa protein antibody; Androgen receptor-associated protein of 70 kDa antibody; ARA 70 antibody; ARA70 antibody; DKFZp762E1112 antibody; ELE 1 antibody; ELE1 antibody; ELE1/ret TK antibody; NCOA 4 antibody; NCoA-4 antibody; NCOA4 antibody; NCOA4_HUMAN antibody; Nuclear receptor coactivator 4 antibody; Papillary thyroid carcinoma 3 antibody; PTC 3 antibody; PTC3 antibody; RET activating gene ELE1 antibody; Ret activating protein ELE1 antibody; Ret-activating protein ELE1 antibody; RFG antibody)
Customer Review

Based on 1 Customer Validation

NCOA4 Antibody (YA4630) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCOA4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for NCOA4 Antibody (YA4630)
  • NCOA4 Antibody (YA4630) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:1000-1:5000

Product Details

Description

NCOA4 Antibody (YA4630) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NCOA4.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Species Reactivity Database

Entrez Gene: 8031 Human

SwissProt: Q13772 Human

Immunogen

Recombinant protein within human NCOA4 aa 1-400 / 614.

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719008

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for NCOA4 Antibody (YA4630)
    Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    NCOA4 is a Cargo receptor for the autophagic turnover of the iron-binding ferritin complex, playing a central role in iron homeostasis. Acts as an adapter for delivery of ferritin to lysosomes and autophagic degradation of ferritin, a process named ferritinophagy. Targets the iron-binding ferritin complex to autolysosomes following starvation or iron depletion. Ensures efficient erythropoiesis, possibly by regulating hemin-induced erythroid differentiation. In some studies, has been shown to enhance the androgen receptor AR transcriptional activity as well as acting as ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma. Another study shows only weak behavior as a coactivator for the androgen receptor and no alteration of the ligand responsiveness of the AR. Binds to DNA replication origins, binding is not restricted to sites of active transcription and may likely be independent from the nuclear receptor transcriptional coactivator function. May inhibit activation of DNA replication origins, possibly by obstructing DNA unwinding via interaction with the MCM2-7 complex[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasmic vesicle, autophagosome; Autolysosome; Nucleus; Chromosome

  • Expression


    Tissue_specificity:Widely expressed. Detected in adipose tissue and various cell lines. The β isoform is expressed only in the testes.

    Induction:Down-regulated by nutrient deprivation (at protein level)

    Positive sample: HeLa cell lysate, A-172 cell lysate, A549 cell lysate, A431 cell lysate, HL-60 cell lysate, NIH:OVCAR-3 cell lysate, A375 cell lysate.

  • Isoforms & Post-Translational Modification

    Q13772 has 4 isomers: Q13772-1: 69726 Da (predicted); Q13772-2: 32562 Da (predicted); Q13772-3: 71371 Da (predicted); Q13772-4: 73649 Da (predicted).
    NCOA4 levels may be regulated via HERC2-mediated ubiquitination leading to proteosomal degradation; HERC2 association is dependent on NCOA4 iron occupancy

  • Subunit

    Interacts with the ferritin complex, an oligomeric structure composed of varying combinations of ferritin heavy chains and ferritin light chains (PubMed:25327288). Interacts (via C-terminus) with ferritin heavy chain FTH1 (via 'Arg-23'); thereby targeting the iron-binding ferritin complex to autolysosomes following starvation or iron depletion (PubMed:25327288, PubMed:26436293). Interacts with ferritin light chain FTL (PubMed:25327288). Interacts (via C-terminus) with ATG8-like proteins GABARAP and GABARAPL1; thereby mediating localization to autophagosomes and ferritinophagy (By similarity). Interacts with MAP1LC3C/LC3C and GABARAPL2 (By similarity). Interacts with the androgen receptor AR (via NR DNA binding domain and NR LBD) in a ligand-dependent manner (PubMed:10517667, PubMed:15563469, PubMed:8643607). Interacts with the retinoid acid receptor RXR-gamma (RXRG) in a ligand-dependent manner (PubMed:10347167). Interacts (via N-terminus) with MCM7 (PubMed:24910095). Interacts with MCM3 (PubMed:24910095). Interacts with CDC45 (PubMed:24910095)

  • SwissProt ID

    Q13772

  • Gene ID
    8031 [NCBI]
  • Synonyms

    70 kDa androgen receptor activator antibody; 70 kDa androgen receptor coactivator antibody; 70 kDa AR activator antibody; 70 kDa AR-activator antibody; Androgen receptor coactivator 70 kD antibody; Androgen receptor coactivator 70 kDa protein antibody; Androgen receptor-associated protein of 70 kDa antibody; ARA 70 antibody; ARA70 antibody; DKFZp762E1112 antibody; ELE 1 antibody; ELE1 antibody; ELE1/ret TK antibody; NCOA 4 antibody; NCoA-4 antibody; NCOA4 antibody; NCOA4_HUMAN antibody; Nuclear receptor coactivator 4 antibody; Papillary thyroid carcinoma 3 antibody; PTC 3 antibody; PTC3 antibody; RET activating gene ELE1 antibody; Ret activating protein ELE1 antibody; Ret-activating protein ELE1 antibody; RFG antibody

NCOA4 Antibody (YA4630) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NCOA4 Antibody (YA4630) Product Image
    1/16
  • Experimental Validation Results for NCOA4 Antibody (YA4630)
  • NCOA4 Antibody (YA4630) Product Image
  • Experimental Validation Results for NCOA4 Antibody (YA4630)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for NCOA4 Antibody (YA4630)
  • NCOA4 Antibody (YA4630) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.Western blot analysis of extracts from HL-60(lane 2(20ug) , A549(lane 3(20ug) and A375(lane 4(20ug) using NCOA4 Antibody (HY-P84934) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • NCOA4 Antibody (YA4630) Product Image WB