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hnRNP G Antibody (YA3029)

(Synonyms: RBMX; HNRPG; RBMXP1; RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G)
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Based on 1 Customer Validation

hnRNP G Antibody (YA3029) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP G.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for hnRNP G Antibody (YA3029)
  • hnRNP G Antibody (YA3029) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

hnRNP G Antibody (YA3029) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to hnRNP G.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 27316 Human

SwissProt: P38159 Human

Immunogen

A synthetic peptide of human hnRNP G aa341-354.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105398

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for hnRNP G Antibody (YA3029)
    Western blot analysis of extracts from HeLa (lane 2(20μg)), Jurkat (lane 3(20μg)), K562 (lane 4(20μg)) and A431 (lane 5(20μg)) using hnRNP G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    hnRNP G is a RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Also plays a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:Expressed strongly in oral keratinocytes, but only weakly detected in oral squamous cell carcinomas (at protein level)

  • Isoforms & Post-Translational Modification

    P38159 has 3 isomers: P38159-1: 42332 Da (predicted); P38159-2: 40846 Da (predicted); P38159-3: 22172 Da (predicted).
    O-glycosylated;Arg-185 is dimethylated, probably to asymmetric dimethylarginine;Cleavage of initiator Met is partial. If Met-1 is not removed, it is acetylated. If it is removed, Val-2 is acetylated

  • Subunit

    Homomultimer. Found in the supraspliceosome complex. Interacts with PPIA/CYPA (PubMed:25678563)

  • SwissProt ID

    P38159

  • Gene ID
    27316 [NCBI]
  • Synonyms

    RBMX; HNRPG; RBMXP1; RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G

  • Research Field

    Epigenetics and Nuclear Signaling

hnRNP G Antibody (YA3029) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg)), Jurkat (lane 3(20μg)), K562 (lane 4(20μg)) and A431 (lane 5(20μg)) using hnRNP G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • hnRNP G Antibody (YA3029) Product Image
    1/16
  • Experimental Validation Results for hnRNP G Antibody (YA3029)
  • hnRNP G Antibody (YA3029) Product Image
  • Experimental Validation Results for hnRNP G Antibody (YA3029)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg)), Jurkat (lane 3(20μg)), K562 (lane 4(20μg)) and A431 (lane 5(20μg)) using hnRNP G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for hnRNP G Antibody (YA3029)
  • hnRNP G Antibody (YA3029) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg)), Jurkat (lane 3(20μg)), K562 (lane 4(20μg)) and A431 (lane 5(20μg)) using hnRNP G Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (beta Actin, HY-P83730, 1/5000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • hnRNP G Antibody (YA3029) Product Image WB