PLZF Antibody (YA4186)(PBS only)

Cat. No.: HY-P84489A: Data Sheet User Guide for Antibodies
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PLZF Antibody (YA4186) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PLZF.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PLZF Antibody (YA4186) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PLZF.

Host

Mouse

Molecular Weight

Predicted band size: 74 kDa;

Observed band size: 74 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q05516

Gene ID

7704 [NCBI]

Immunogen

Purified recombinant fragment of human ZBTB16 aa 1-254.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as a transcriptional repressor (PubMed:10688654, PubMed:24359566). Transcriptional repression may be mediated through recruitment of histone deacetylases to target promoters (PubMed:10688654). May play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues. Probable substrate-recognition component of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:14528312)

Subcellular Localization：Nucleus; Nucleus, nuclear body

Expression：
Tissue_specificity:In the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines, and peripheral blood mononuclear cells. It is also expressed in the ovary, with lower levels in the kidney and lung.

Induction:By retinoic acid

Isoforms & Post-Translational Modification：Q05516 has 2 isomers: Q05516-1: 74274 Da (predicted); Q05516-2: 61601 Da (predicted).

Subunit：Binds EPN1 (PubMed:11161217). Interacts with ZBTB32 and CUL3 (PubMed:10572087, PubMed:14528312). Interacts with ATP7B (PubMed:16676348). Interacts with transcriptional corepressor RUNX1T1 (via its N-terminus); the interaction increases the transcription repression activity of ZBTB16 (PubMed:10688654). Interacts (via C2H2-type zinc finger domains 1 and 2) with RNF112 (PubMed:24359566)

Synonyms

PLZF; ZNF145; ZBTB16

Documentation

User Guide for Antibodies (1077 KB)