NFE2L1 Antibody (YA7030)

Cat. No.: HY-P87347: Data Sheet User Guide for Antibodies
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NFE2L1 Antibody (YA7030) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NFE2L1.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

NFE2L1 Antibody (YA7030) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to NFE2L1.

Host

Mouse

Clonality

Monoclonal

분자량

Predicted band size: 30kDa;

Observed band size: 30kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q14494

Immunogen

Amino acids 191-475 of Nrf1 of human origin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1:1000
IP IP: Immunoprecipitation	1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:500

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

선적

Shipping with blue ice.

Background

Function：Endoplasmic reticulum membrane sensor that translocates into the nucleus in response to various stresses to act as a transcription factor (PubMed:20932482, PubMed:24448410). Constitutes a precursor of the transcription factor NRF1 (By similarity). Able to detect various cellular stresses, such as cholesterol excess, oxidative stress or proteasome inhibition (PubMed:20932482). In response to stress, it is released from the endoplasmic reticulum membrane following cleavage by the protease DDI2 and translocates into the nucleus to form the transcription factor NRF1 (By similarity). Acts as a key sensor of cholesterol excess: in excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal, such as CD36 (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, it is released from the endoplasmic reticulum membrane, translocates to the nucleus and activates expression of genes encoding proteasome subunits (PubMed:20932482)|CNC-type bZIP family transcription factor that translocates to the nucleus and regulates expression of target genes in response to various stresses (PubMed:8932385, PubMed:9421508). Heterodimerizes with small-Maf proteins (MAFF, MAFG or MAFK) and binds DNA motifs including the antioxidant response elements (AREs), which regulate expression of genes involved in oxidative stress response (PubMed:8932385, PubMed:9421508). Activates or represses expression of target genes, depending on the context (PubMed:8932385, PubMed:9421508). Plays a key role in cholesterol homeostasis by acting as a sensor of cholesterol excess: in low cholesterol conditions, translocates into the nucleus and represses expression of genes involved in defense against cholesterol excess, such as CD36 (By similarity). In excess cholesterol conditions, the endoplasmic reticulum membrane form of the protein directly binds cholesterol via its CRAC motif, preventing cleavage and release of the transcription factor NRF1, thereby allowing expression of genes promoting cholesterol removal (By similarity). Critical for redox balance in response to oxidative stress: acts by binding the AREs motifs on promoters and mediating activation of oxidative stress response genes, such as GCLC, GCLM, GSS, MT1 and MT2 (By similarity). Plays an essential role during fetal liver hematopoiesis: probably has a protective function against oxidative stress and is involved in lipid homeostasis in the liver (By similarity). Involved in proteasome homeostasis: in response to proteasome inhibition, mediates the 'bounce-back' of proteasome subunits by translocating into the nucleus and activating expression of genes encoding proteasome subunits (PubMed:20932482). Also involved in regulating glucose flux (By similarity). Together with CEBPB; represses expression of DSPP during odontoblast differentiation (PubMed:15308669). In response to ascorbic acid induction, activates expression of SP7/Osterix in osteoblasts

Subcellular Localization：Endoplasmic reticulum membrane,Endoplasmic reticulum membrane,Nucleus

Isoforms & Post-Translational Modification：Q14494 has two isomers: Q14494-1: 84704 Da (predicted); Q14494-2: 81499 Da (predicted).
Cleaved at Leu-104 by the aspartyl protease DDI2 following retrotranslocation, releasing the protein from the endoplasmic reticulum membrane and forming the transcription factor NRF1 that translocates into the nucleus (PubMed:24448410, PubMed:27528193, PubMed:27676297, PubMed:27676298)丨N-glycosylated in normal conditions, when it has a single-pass type II membrane protein topology, with the DNA-binding domain facing the endoplasmic reticulum lumen (PubMed:20932482, PubMed:24448410, PubMed:24998528, PubMed:27528193)丨Ubiquitinated by the SCF(FBXW7) complex and SYVN1/HRD1, leading to its degradation by the proteasome (PubMed:20932482)丨Phosphorylation by CK2 at Ser-528 inhibits transcription factor activity, possibly by affecting DNA-binding activity (By similarity)丨Ubiquitinated by the SCF(BTRC) complex in the nucleus, leading to its degradation by the proteasome

Subunit：Interacts with KEAP1

Synonyms

erythroid derived 2 antibody; like 1 antibody; Locus control region factor 1 antibody; Locus control region-factor 1 antibody; NF-E2-related factor 1 antibody; NF2L1_HUMAN antibody; NFE2 related factor 1 antibody; NFE2-related factor 1 antibody; NFE2L1 antibody; NRF1 antibody

각종 서류

User Guide for Antibodies (1077 KB)