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ERK1 Antibody (YA454)

(Synonyms: ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK)
Customer Review

Based on 1 Customer Validation

ERK1 Antibody (YA454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • ERK1 Antibody (YA454) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:100-1:500 1:50-1:200 1:50-1:100

Product Details

Description

ERK1 Antibody (YA454) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ERK1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database
Immunogen

Synthetic peptide within N-terminal human ERK1.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102476

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ERK1 Antibody (YA454)
    Western blot analysis of extracts from M-heart (lane 1) and M-spleen (lane 2) using ERK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for ERK1 Antibody (YA454)
    Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ERK1 Antibody (YA454)
    Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ERK1 (MAPK3) is a serine/threonine protein kinase that functions as an essential effector of the MAPK/ERK signaling cascade and transduces extracellular signals controlling cell proliferation, differentiation, migration, and survival[1][2]. Mechanistically, ERK1 operates downstream of the Ras/Raf/MEK pathway, where dual phosphorylation activates ERK signaling and enables phosphorylation of numerous substrates across multiple cellular compartments, thereby regulating gene expression and diverse physiological processes[2]. Because the MAPK/ERK pathway integrates growth factor, hormone, and stress-associated signals, dysregulated ERK signaling contributes to pathological conditions including cancer and other diseases characterized by aberrant cellular proliferation and survival signaling[1]. In experimental systems, ERK1 has been extensively investigated in developmental, vascular, and disease models to define its contribution to signal transduction and tissue responses[2][3]. Compared with the closely related isoform ERK2 (MAPK1)， ERK1 exhibits highly conserved activation mechanisms, substrate recognition properties, and signaling outputs, and current evidence generally supports substantial functional redundancy between the two kinases[2]. Nevertheless, isoform-specific studies have identified distinct biological contexts in which ERK1 and ERK2 contribute differently to physiological processes, including vascular remodeling and arteriogenesis, indicating that isoform-dependent functions can emerge in specific cellular environments[3]. For experimental applications, pharmacological ERK1/2 inhibitors are widely used to interrogate terminal MAPK pathway signaling and to investigate mechanisms of therapeutic resistance associated with reactivation of ERK signaling downstream of RAF or MEK inhibition[1].

  • Subcellular Localization

    Cytoplasm; Nucleus; Membrane, caveola; Cell junction, focal adhesion

  • Subunit

    Binds both upstream activators and downstream substrates in multimolecular complexes. Found in a complex with at least BRAF, HRAS, MAP2K1/MEK1, MAPK3 and RGS14 (By similarity). Interacts with ADAM15, ARRB2, CANX, DAPK1 (via death domain), HSF4, IER3, MAP2K1/MEK1, MORG1, NISCH, and SGK1. Interacts with PEA15 and MKNK2 (By similarity). MKNK2 isoform 1 binding prevents from dephosphorylation and inactivation (By similarity). Interacts with TPR. Interacts with CDKN2AIP. Interacts with HSF1 (via D domain and preferentially with hyperphosphorylated form); this interaction occurs upon heat shock (PubMed:10747973). Interacts with CAVIN4 (By similarity). Interacts with GIT1; this interaction is necessary for MAPK3 localization to focal adhesions (By similarity). Interacts with ZNF263 (PubMed:32051553). Interacts with EBF4

  • SwissProt ID

    P27361

  • Gene ID
    5595 [NCBI]
  • Synonyms

    ERK1, PRKM3, MAPK3, Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, Microtubule-associated protein 2 kinase, p44-ERK1, ERK-1, p44-MAPK

  • Research Field

    Cell Biology

[1]. Sah VK, et al. Advances in ERK1/2 inhibition: a medicinal chemistry perspective on structure and regulation. J Enzyme Inhib Med Chem. 2025 Dec;40(1):2555510. [Content Brief]

[2]. Buscà R, et al. ERK1 and ERK2 Map Kinases: Specific Roles or Functional Redundancy? Front Cell Dev Biol. 2016 Jun 8;4:53. [Content Brief]

[3]. Ricard N, et al. Isoform-Specific Roles of ERK1 and ERK2 in Arteriogenesis. Cells. 2019 Dec 21;9(1):38. [Content Brief]

ERK1 Antibody (YA454) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-heart (lane 1) and M-spleen (lane 2) using ERK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ERK1 Antibody (YA454) Product Image
    1/16
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • ERK1 Antibody (YA454) Product Image
  • Experimental Validation Results for ERK1 Antibody (YA454)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-heart (lane 1) and M-spleen (lane 2) using ERK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for ERK1 Antibody (YA454)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ERK1 Antibody (YA454)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • Experimental Validation Results for ERK1 Antibody (YA454)
  • ERK1 Antibody (YA454) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-heart (lane 1) and M-spleen (lane 2) using ERK1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • ERK1 Antibody (YA454) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ERK1 Antibody (YA454) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling ERK1 with ERK1 Antibody (HY-P80391) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ERK1 Antibody (HY-P80391) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ERK1 Antibody (YA454) Product Image WB
  • ERK1 Antibody (YA454) Product Image ICC
  • ERK1 Antibody (YA454) Product Image ICC