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Wee1 Antibody (YA6840)

(Synonyms: DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; Wee1 homolog antibody; WEE1 homolog S. pombe antibody; Wee1 like protein kinase antibody; Wee1 tyrosine kinase antibody; Wee1+ homolog antibody; Wee1+ S. pombe homolog antibody; WEE1, S. pombe, homolog of antibody; WEE1, somatic antibody; Wee1-like protein kinase antibody; WEE1_HUMAN antibody; WEE1A antibody; Wee1A kinase antibody; WEE1hu antibody; )
Customer Review

Based on 1 Customer Validation

Wee1 Antibody (YA6840) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wee1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Monkey

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Wee1 Antibody (YA6840) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:200

Product Details

Description

Wee1 Antibody (YA6840) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wee1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Monkey
  • Observed Molecular Weight
    Observed band size: 90 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 72 kDa
Immunogen

Recombinant protein within human Wee1 aa 251-600 / 646.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), HT-29 (lane4(20μg) and Jurkat (lane5(20μg) using Wee1 Antibody (HY-P87147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human breast tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Wee1 is a nuclear kinase belonging to the Ser/Thr family of protein kinases in the fission yeast Schizosaccharomyces pombe (S. pombe) . Wee1 has amolecular mass of 96 kDa and it is a key regulator of cell cycle progression. Wee1 influences cell size by inhibiting the entry into mitosis, through inhibiting Cdk1. Wee1 has homologues in many other organisms, including mammals. Wee1 inhibits Cdk1 by phosphorylating it on two different sites, Tyr15 and Thr14. Cdk1 is crucial for the cyclin-dependent passage of the various cell cycle checkpoints. At least three checkpoints exist for which the inhibition of Cdk1 by Wee1 is important: G2/M checkpoint, Cell size checkpoint, DNA damage checkpoint. Wee1 is shown to phosphorylate histone H2B at tyrosine 37 residue which regulates global expression of histones.
    WEE1 subfamily includes PMYT1.

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P30291 has two isomers: P30291-1: 71597 Da (predicted); P30291-2: 49072 Da (predicted).
    Phosphorylated during M and G1 phases丨Dephosphorylated at Thr-239 by CTDP1 (PubMed:22692537)丨Ubiquitinated and degraded at the onset of G2/M phase

  • SwissProt ID

    P30291

  • Gene ID
    7465 [NCBI]
  • Synonyms

    DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; DKFZp686I18166 antibody; EC 2.7.10.2 antibody; FLJ16446 antibody; MGC105683 antibody; OTTHUMP00000231338 antibody; OTTHUMP00000231339 antibody; Wee 1 antibody; WEE 1 homolog 1 (S. pombe) antibody; WEE1 antibody; WEE1 homolog (S. pombe) antibody; Wee1 homolog antibody; WEE1 homolog S. pombe antibody; Wee1 like protein kinase antibody; Wee1 tyrosine kinase antibody; Wee1+ homolog antibody; Wee1+ S. pombe homolog antibody; WEE1, S. pombe, homolog of antibody; WEE1, somatic antibody; Wee1-like protein kinase antibody; WEE1_HUMAN antibody; WEE1A antibody; Wee1A kinase antibody; WEE1hu antibody;

Wee1 Antibody (YA6840) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), HT-29 (lane4(20μg) and Jurkat (lane5(20μg) using Wee1 Antibody (HY-P87147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    1/16
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Wee1 Antibody (YA6840) Product Image
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), HT-29 (lane4(20μg) and Jurkat (lane5(20μg) using Wee1 Antibody (HY-P87147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human cervix tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Experimental Validation Results for Wee1 Antibody (YA6840)
  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), Raji (lane3(20μg), HT-29 (lane4(20μg) and Jurkat (lane5(20μg) using Wee1 Antibody (HY-P87147). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human cervix tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Wee1 Antibody (HY-P87147, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Wee1 Antibody (YA6840) Product Image WB
  • Wee1 Antibody (YA6840) Product Image IHC-P
  • Wee1 Antibody (YA6840) Product Image IHC-P
  • Wee1 Antibody (YA6840) Product Image IHC-P
  • Wee1 Antibody (YA6840) Product Image IHC-P
  • Wee1 Antibody (YA6840) Product Image IHC-P
  • Wee1 Antibody (YA6840) Product Image IHC-P