Stromal Interaction Molecule 1 Antibody (YA6222)
(Synonyms: Stromal interaction molecule 1)
Based on 1 Customer Validation
Stromal Interaction Molecule 1 Antibody (YA6222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Stromal Interaction Molecule 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
Stromal Interaction Molecule 1 Antibody (YA6222) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Stromal Interaction Molecule 1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 77 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 77 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HeLa (lane 1(20μg)) 、K562 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) 、RAW264.7 (lane 4(20μg)) and PC-12 (lane 5(20μg)) using Stromal Interaction Molecule 1 Antibody (HY-P86530) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
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Function
Stromal Interaction Molecule 1 acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Endoplasmic reticulum membrane; Single-pass type I membrane protein; Cytoplasm, cytoskeleton; Sarcoplasmic reticulum
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Expression
Tissue_specificity:It is widely expressed in a variety of primary human cells and tumor cell lines. -
Isoforms & Post-Translational Modification
Q13586 has 2 isomers: Q13586-1: 77423 Da (predicted); Q13586-2: 62133 Da (predicted).
Glycosylation is required for cell surface expression;Phosphorylated predominantly on Ser residues -
Subunit
Monomer in the presence of Ca(2+); it oligomerizes in absence of Ca(2+) (PubMed:18854159). Forms homooligomers and heterooligomers with STIM2 (PubMed:11463338, PubMed:11983428, PubMed:18854159, PubMed:22451904, PubMed:24069340, PubMed:24351972). Interacts with pore-forming subunits of CRAC channels, ORAI1, ORAI2 and ORAI3; this interaction is potentiated upon Ca(2+) store depletion (PubMed:19706554, PubMed:32415068). Interacts (via the transmembrane region and the SOAR/CAD domain) with SPPL3; the interaction promotes the binding of STIM1 to ORAI1 (PubMed:25384971). Interacts (via the SOAR/CAD domain) witH2O2AI1 (PubMed:17905723, PubMed:19182790, PubMed:19249086, PubMed:24351972, PubMed:27185316, PubMed:28219928, PubMed:30481768). Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends (PubMed:19632184). Interacts with CRACR2A/EFCAB4B; the interaction is direct and takes place in absence of Ca(2+) (PubMed:20418871). Forms a complex with CRACR2A/EFCAB4B and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations (PubMed:20418871). Interacts with SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1 (PubMed:22464749, PubMed:30481768). Interacts with EFHB; the interaction takes place upon Ca(2+)-store depletion and inhibits the association with SARAF (PubMed:30481768). Interacts with ASPH (isoform 8) (PubMed:22586105). Interacts with SLC35G1; intracellular Ca(2+)-dependent. May interact with ATP1A1, ATP2A2, ATP2B1, ATP2B4, KPNB1 and XPO1; tH2O2gh SLC35G1 (PubMed:22084111). Interacts with TMEM203 (PubMed:25996873). Interacts with STIMATE, promoting STIM1 conformational switch (PubMed:26322679). Interacts with TMEM178A (By similarity). Interacts with CASQ1 (via C-terminal end and preferentially with the monomeric form); this interaction increases in response to a depletion of intracellular Ca(2+), decreases both STIM1 aggregation and clustering, interaction of STIM1 witH2O2AI1 and store-operated Ca(2+) entry (SOCE) activity (PubMed:27185316). Interacts with ADCY8 (PubMed:22494970)
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SwissProt ID
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Synonyms
Stromal interaction molecule 1
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