HIF Prolyl Hydroxylases Antibody (YA2975)(PBS only)

Cat. No.: HY-P83230A: Data Sheet User Guide for Antibodies
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HIF Prolyl Hydroxylases Antibody (YA2975) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HIF Prolyl Hydroxylases.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

HIF Prolyl Hydroxylases Antibody (YA2975) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HIF Prolyl Hydroxylases.

Host

Rabbit

분자량

Predicted band size: 57 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NXG6

Gene ID

54681 [NCBI]

Immunogen

A synthetic peptide of human HIF Prolyl Hydroxylases

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type II membrane protein

Expression：
Tissue_specificity:Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts

Induction:By hypoxia in many cultured cell lines

Isoforms & Post-Translational Modification：Q9NXG6 has 3 isomers: Q9NXG6-1: 56661 Da (predicted); Q9NXG6-2: 47211 Da (predicted); Q9NXG6-3: 63112 Da (predicted).
Glycosylated

Subunit：Homodimer

Research Field

Cardiovascular

Synonyms

PH4; PH-4; PHD4; EGLN4; HIDEA; HIFPH4; P4H-TM

각종 서류

User Guide for Antibodies (1077 KB)