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TAF15 Antibody (YA2134)

(Synonyms: TAF15; RBP56; TAF2N; TATA-binding protein-associated factor 2N; 68 kDa TATA-binding protein-associated factor; TAF(II)68; TAFII68; RNA-binding protein 56)
Customer Review

Based on 1 Customer Validation

TAF15 Antibody (YA2134) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAF15.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • TAF15 Antibody (YA2134) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

TAF15 Antibody (YA2134) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TAF15.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 77 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human TAF15 aa579-592.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TAF15 Antibody (YA2134)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Caco-2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and C6 (lane 5, 15 μg) using TAF15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    TAF15 is a RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Binds to ssRNA containing the consensus sequence 5'-AGGUAA-3'. Can enter the preinitiation complex together with the RNA polymerase II (Pol II)[1].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Ubiquitous. Observed in all human tissues

  • Isoforms & Post-Translational Modification

    Q92804 has 2 isomers: Q92804-1: 61830 Da (predicted); Q92804-2: 61558 Da (predicted).
    Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity;ADP-ribosylated during genotoxic stress

  • Subunit

    Belongs to the RNA polymerase II (Pol II) transcriptional multiprotein complex, together with the TATA-binding protein (TBP) and other TBP-associated factors (TAF(II)s). Binds SF1

  • SwissProt ID

    Q92804

  • Gene ID
    8148 [NCBI]
  • Synonyms

    TAF15; RBP56; TAF2N; TATA-binding protein-associated factor 2N; 68 kDa TATA-binding protein-associated factor; TAF(II)68; TAFII68; RNA-binding protein 56

  • Research Field

    Epigenetics and Nuclear Signaling

TAF15 Antibody (YA2134) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Caco-2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and C6 (lane 5, 15 μg) using TAF15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TAF15 Antibody (YA2134) Product Image
    1/16
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • TAF15 Antibody (YA2134) Product Image
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Caco-2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and C6 (lane 5, 15 μg) using TAF15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • Experimental Validation Results for TAF15 Antibody (YA2134)
  • TAF15 Antibody (YA2134) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), 293T (lane 2, 15 μg), Caco-2 (lane 3, 15 μg), Jurkat (lane 4, 15 μg), and C6 (lane 5, 15 μg) using TAF15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:2000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • TAF15 Antibody (YA2134) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TAF15 Antibody (YA2134) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TAF15 Antibody (YA2134) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TAF15 Antibody (YA2134) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using TAF15 Antibody (YA2134). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P82389, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • TAF15 Antibody (YA2134) Product Image WB
  • TAF15 Antibody (YA2134) Product Image IHC-P
  • TAF15 Antibody (YA2134) Product Image IHC-P
  • TAF15 Antibody (YA2134) Product Image IHC-P
  • TAF15 Antibody (YA2134) Product Image IHC-P