ING4 Antibody
(Synonyms: My036, ING4, Inhibitor of growth protein 4, p29ING4)ING4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ING4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
ING4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ING4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 29 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 20; 25; 28 kDa
Purified recombinant protein of human ING4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ING4 is a Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity)[1][2][3][4][5].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
ING4 has 8 isoforms, Q9UNL4-1: amino acid length is 249, molecular weight is 28530 Da (predicted); Q9UNL4-2: amino acid length is 248, molecular weight is 28432 Da (predicted); Q9UNL4-3: amino acid length is 225, molecular weight is 25554 Da (predicted); Q9UNL4-4: amino acid length is 245, molecular weight is 28089 Da (predicted); Q9UNL4-5: amino acid length is 246, molecular weight is 28218 Da (predicted); Q9UNL4-6: amino acid length is 248, molecular weight is 28402 Da (predicted); Q9UNL4-7: amino acid length is 246, molecular weight is 28217 Da (predicted); Q9UNL4-8: amino acid length is 179, molecular weight is 20482 Da (predicted).核定位信号内的位点被 PADI4 瓜氨酸化后，会破坏其与 p53 的相互作用，并增加其降解易感性ING4 存在 8 个异构体，Q9UNL4-1：氨基酸个数为 249 个，分子量为 28530 Da (预测)；Q9UNL4-2：氨基酸个数为 248 个，分子量为 28432 Da (预测)；Q9UNL4-3：氨基酸个数为 225 个，分子量为 25554 Da (预测)；Q9UNL4-4：氨基酸个数为 245 个，分子量为 28089 Da (预测)；Q9UNL4-5：氨基酸个数为 246 个，分子量为 28218 Da (预测)；Q9UNL4-6：氨基酸个数为 248 个，分子量为 28402 Da (预测)；Q9UNL4-7：氨基酸个数为 246 个，分子量为 28217 Da (预测)；Q9UNL4-8：氨基酸个数为 179 个，分子量为 20482 Da (预测)。Citrullination by PADI4 within the nuclear localization signal disrupts the interaction with p53 and increases susceptibility to degradation
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
My036, ING4, Inhibitor of growth protein 4, p29ING4
Documentation
[1]. Doyon Y, et al. ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation. Mol Cell. 2006 Jan 6;21(1):51-64. [Content Brief]
[2]. Zhang X, et al. ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells. FEBS Lett. 2004 Jul 16;570(1-3):7-12. [Content Brief]
[3]. Kim S, et al. A screen for genes that suppress loss of contact inhibition: identification of ING4 as a candidate tumor suppressor gene in human cancer. Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16251-6. [Content Brief]
[4]. Garkavtsev I, et al. The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis. Nature. 2004 Mar 18;428(6980):328-32. [Content Brief]
[5]. Ozer A, et al. The candidate tumor suppressor ING4 represses activation of the hypoxia inducible factor (HIF). Proc Natl Acad Sci U S A. 2005 May 24;102(21):7481-6. [Content Brief]
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