Caspase-5 p20 Antibody (YA7715)

Cat. No.: HY-P88030: Data Sheet User Guide for Antibodies
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Caspase-5 p20 Antibody (YA7715) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-5 p20.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Caspase-5 p20 Antibody (YA7715) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-5 p20.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 50 kDa;

Observed band size: 47 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P51878

Immunogen

A synthesized peptide derived from human Caspase 5

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Thiol protease that acts as a mediator of programmed cell death (PubMed:28314590, PubMed:29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893). Also mediates cleavage and maturation of IL18 (PubMed:37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed:37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590)

Expression：
Tissue_Specificity: Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle
Induction: Up-regulated by bacterial lipopolysaccharides (LPS)

Isoforms & Post-Translational Modification：P51878 has 6 isomers: P51878-1: 49736 Da (predicted); P51878-2: 43261 Da (predicted); P51878-3: 33304 Da (predicted); P51878-4: 12694 Da (predicted); P51878-5: 51177 Da (predicted); P51878-6: 41851 Da (predicted).
The two subunits are derived from the precursor sequence by an autocatalytic mechanism

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits (By similarity)

Documentation

User Guide for Antibodies (1077 KB)