Phospho-Phospholamban (Ser16) Antibody (YA1618)(PBS only)

Cat. No.: HY-P81873A: Data Sheet User Guide for Antibodies
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Phospho-Phospholamban (Ser16) Antibody (YA1618) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Phospholamban (Ser16).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Phospho-Phospholamban (Ser16) Antibody (YA1618) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Phospholamban (Ser16).

Host

Rabbit

Molecular Weight

Predicted band size: 6 kDa;

Observed band size: 12,24 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P26678

Gene ID

5350 [NCBI]

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser16 of human Phospholamban

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Reversibly inhibits the activity of ATP2A2/SERCA2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+) (PubMed:28890335). Binds preferentially to the ATP-bound E1 conformational form of ATP2A2 which predominates at low Ca(2+) concentrations during the diastolic phase of the cardiac cycle (By similarity). Inhibits ATP2A2 Ca(2+) affinity by disrupting its allosteric activation by ATP (By similarity). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity). Also inhibits the activity of ATP2A3/SERCA3 (By similarity). Controls intracellular Ca(2+) levels in elongated spermatids and may play a role in germ cell differentiation (By similarity). In the thalamic reticular nucleus of the brain, plays a role in the regulation of sleep patterns and executive functioning (By similarity)

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass membrane protein; Sarcoplasmic reticulum membrane; Single-pass membrane protein; Mitochondrion membrane; Single-pass membrane protein; Membrane; Single-pass membrane protein

Expression：
Tissue_specificity:Cardiac muscle (protein levels)

Subunit：Homopentamer (PubMed:16043693, PubMed:16897780). Can also form heterooligomers witH2O2her sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA) regulators ARLN, ERLN, SLN and STRIT1/DWORF (PubMed:36523160).

Research Field

Signal Transduction

Synonyms

PLN; PLB; Cardiac phospholamban; PLB

Documentation

User Guide for Antibodies (1077 KB)