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Glucocorticoid Receptor Antibody (YA412)

(Synonyms: GR; GCR; GRL; GCCR; GCRST)
Customer Review

Based on 1 Customer Validation

Glucocorticoid Receptor Antibody (YA412) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucocorticoid Receptor.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Glucocorticoid Receptor Antibody (YA412) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Glucocorticoid Receptor Antibody (YA412) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glucocorticoid Receptor.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 94/91 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 87 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Mouse Glucocorticoid Receptor full length.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102666

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    Western blot analysis of extracts from HL-60 (lane 2, 20ug), A549 (lane 3, 20ug), THP-1 (lane 4, 20ug) and 3T3-L1 (lane 5, 20ug) using Glucocorticoid Receptor Antibody (HY-P80687). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    Immunocytochemistry analysis of NIH/3T3 cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Glucocorticoid Receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is the receptor to which cortisol and other glucocorticoids bind. The GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. When the glucocorticoid receptor binds to glucocorticoids, its primary mechanism of action is the regulation of gene transcription. The unbound receptor resides in the cytosol of the cell. After the receptor is bound to glucocorticoid, the receptor-glucorticoid complex can take either of two paths. The activated GR complex up-regulates the expression of anti-inflammatory proteins in the nucleus or represses the expression of pro-inflammatory proteins in the cytosol by preventing the translocation of other transcription factors from the cytosol into the nucleus. Dexamethasone is an agonist, and RU486 and cyproterone acetate are antagonists of the GR. Also, progesterone and DHEA have antagonist effects on the GR.

  • Subcellular Localization

    Cytoplasm; Nucleus; Mitochondrion; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Chromosome; Nucleus, nucleoplasm; Cytoplasm; Nucleus; Mitochondrion; Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Nucleus; Cytoplasm

  • Expression


    Tissue_specificity:Expressed in spleen, kidney and liver (PubMed:20660300) . Expressed in a circadian manner in the liver (PubMed:27686098) . Isoform 3: Expressed at highest level in spleen with lesser amounts in kidney and liver.

    Induction:Down-regulated by glucocorticoids; Up-regulated by glucocorticoids and insulin

  • Isoforms & Post-Translational Modification

    P06537 has 5 isomers: P06537-1: 87164 Da (predicted); P06537-2: 87320 Da (predicted); P06537-3: 84384 Da (predicted); P06537-4: 84540 Da (predicted); P06537-5: 82999 Da (predicted).
    Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity;Increased proteasome-mediated degradation in response to glucocorticoids;Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoids. Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-221, Ser-243 and Ser-421-phosphorylated forms are mainly cytoplasmic, and the Ser-229-phosphorylated form is nuclear (By similarity). Phosphorylation at Ser-229 increases transcriptional activity (By similarity). Phosphorylation at Ser-221, Ser-243 and Ser-421 decreases signaling capacity (By similarity). Phosphorylation at Ser-421 may protect from glucocorticoid-induced apoptosis (By similarity). Phosphorylation at Ser-221 and Ser-229 is not required in regulation of chromosome segregation (By similarity). May be dephosphorylated by PPP5C, attenuates NR3C1 action (PubMed:21994940);Sumoylation at Lys-294 and Lys-310 negatively regulates its transcriptional activity. Sumoylation at Lys-718 positively regulates its transcriptional activity in the presence of RWDD3. Sumoylation at Lys-294 and Lys-310 is dispensable whereas sumoylation at Lys-718 is critical for the stimulatory effect of RWDD3 on its transcriptional activity. Heat shock increases sumoylation in a RWDD3-dependent manner;Ubiquitinated (PubMed:11555652). Ubiquitination by UBR5 leads to its degradation: UBR5 specifically recognizes and binds ligand-bound NR3C1 when it is not associated with coactivators (NCOAs) (By similarity). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation (By similarity)

  • Subunit

    Heteromultimeric cytoplasmic complex with HSP90AA1, HSPA1A/HSPA1B, and FKBP5 or another immunophilin such as PPID, STIP1, or the immunophilin homolog PPP5C (PubMed:21994940, PubMed:9195923).

  • SwissProt ID

    P06537

  • Gene ID
    2908 [NCBI]
  • Synonyms

    GR; GCR; GRL; GCCR; GCRST

  • Research Field

    Signal Transduction

Glucocorticoid Receptor Antibody (YA412) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HL-60 (lane 2, 20ug), A549 (lane 3, 20ug), THP-1 (lane 4, 20ug) and 3T3-L1 (lane 5, 20ug) using Glucocorticoid Receptor Antibody (HY-P80687). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucocorticoid Receptor Antibody (YA412) Product Image
    1/16
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Glucocorticoid Receptor Antibody (YA412) Product Image
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HL-60 (lane 2, 20ug), A549 (lane 3, 20ug), THP-1 (lane 4, 20ug) and 3T3-L1 (lane 5, 20ug) using Glucocorticoid Receptor Antibody (HY-P80687). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of NIH/3T3 cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Experimental Validation Results for Glucocorticoid Receptor Antibody (YA412)
  • Glucocorticoid Receptor Antibody (YA412) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HL-60 (lane 2, 20ug), A549 (lane 3, 20ug), THP-1 (lane 4, 20ug) and 3T3-L1 (lane 5, 20ug) using Glucocorticoid Receptor Antibody (HY-P80687). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Glucocorticoid Receptor Antibody (YA412) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of NIH/3T3 cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucocorticoid Receptor Antibody (YA412) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Glucocorticoid Receptor Antibody (HY-P80687) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glucocorticoid Receptor Antibody (YA412) Product Image WB
  • Glucocorticoid Receptor Antibody (YA412) Product Image ICC
  • Glucocorticoid Receptor Antibody (YA412) Product Image ICC