ALDH2 Antibody (YA929)
(Synonyms: ALDH2; ALDM; Aldehyde dehydrogenase; mitochondrial; ALDH class 2; ALDH-E2; ALDHI)Based on 1 Customer Validation
ALDH2 Antibody (YA929) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IP
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Reactivity :
Human, Mouse
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IP
IP: Immunoprecipitation
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:20 |
Product Details
ALDH2 Antibody (YA929) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ALDH2.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 56 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 56 kDa
A synthetic peptide of human ALDH2
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from HepG2(lane 2(20ug) ,A549(lane 3(20ug) and THP-1(lane 4(20ug) using ALDH2 Antibody (HY-P81257A) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
ALDH2 (aldehyde dehydrogenase 2) is a mitochondrial NAD (P) +-dependent enzyme that catalyzes the irreversible oxidation of acetaldehyde and other reactive aldehydes into their corresponding carboxylic acids, thereby maintaining cellular detoxification and redox homeostasis[1][2]. Mechanistically, ALDH2 participates in the major oxidative pathway of alcohol metabolism and also eliminates lipid peroxidation-derived aldehydes, including 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA), which accumulate during oxidative stress and mitochondrial dysfunction[3][4]. Through detoxification of these highly reactive aldehydes, ALDH2 limits protein modification, oxidative injury, and disruption of intracellular signaling pathways associated with cardiovascular and metabolic disorders[3][4]. In disease models, reduced ALDH2 activity has been linked to myocardial injury, heart failure, diabetes, neurodegenerative diseases, stroke, and cancer, highlighting its broad role in stress-response mechanisms and tissue protection[4][5]. Compared with related ALDH isoforms, ALDH2 is distinguished by its mitochondrial localization and high catalytic efficiency toward acetaldehyde, whereas ALDH1A family members primarily regulate retinoic acid biosynthesis and ALDH1A1 or ALDH3A1 contribute to ocular protection and other tissue-specific functions[1][2]. A common inactive variant, ALDH22, markedly reduces enzymatic activity and promotes acetaldehyde accumulation, providing a widely used genetic model for studying aldehyde toxicity, oxidative stress, and disease susceptibility in East Asian populations[1]. For experimental applications, both ALDH2-selective activators and inhibitors have been developed to investigate isoform-specific functions, mitochondrial aldehyde metabolism, and therapeutic modulation of oxidative stress pathways[1][5].
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Subcellular Localization
Mitochondrion matrix
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Isoforms & Post-Translational Modification
P05091 has 2 isomers: P05091-1: 56381 Da (predicted); P05091-2: 50989 Da (predicted).
In response to mitochondrial stress, the precursor protein is ubiquitinated by the SIFI complex in the cytoplasm before mitochondrial import, leading to its degradation (PubMed:38297121). Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1 (PubMed:38297121) -
Subunit
Homotetramer
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SwissProt ID
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Synonyms
ALDH2; ALDM; Aldehyde dehydrogenase; mitochondrial; ALDH class 2; ALDH-E2; ALDHI
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Research Field
Tags & Cell Markers
Documentation
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Data Sheet (260 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[1]. Koppaka V, et al. Aldehyde dehydrogenase inhibitors: a comprehensive review of the pharmacology, mechanism of action, substrate specificity, and clinical application. Pharmacol Rev. 2012 Jul;64(3):520-39. [Content Brief]
[2]. ALDH2 gene information from NCBI.
[3]. Xiao P, et al. Edaravone dexborneol protects against cerebral ischemia/reperfusion-induced blood-brain barrier damage by inhibiting ferroptosis via activation of nrf-2/HO-1/GPX4 signaling. Free Radic Biol Med. 2024 May 1;217:116-125. [Content Brief]
[4]. Chen CH, et al. Targeting aldehyde dehydrogenase 2: new therapeutic opportunities. Physiol Rev. 2014 Jan;94(1):1-34. [Content Brief]
[5]. Zhao Y, et al. ALDH2 (Aldehyde Dehydrogenase 2) Protects Against Hypoxia-Induced Pulmonary Hypertension. Arterioscler Thromb Vasc Biol. 2019 Nov;39(11):2303-2319. [Content Brief]
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