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ATP2A1/SERCA1 Antibody (YA3519)

(Synonyms: ATP2A; SERCA1)
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Based on 1 Customer Validation

ATP2A1/SERCA1 Antibody (YA3519) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2A1/SERCA1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human, Mouse, Monkey

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP2A1/SERCA1 Antibody (YA3519)
  • ATP2A1/SERCA1 Antibody (YA3519) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

ATP2A1/SERCA1 Antibody (YA3519) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATP2A1/SERCA1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Monkey
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Immunogen

Purified recombinant fragment of human ATP2A1 (AA: 487-631) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP2A1/SERCA1 Antibody (YA3519)
    Flow cytometric analysis of 1X10^6 Hela cells labeling ATP2A1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    ATP2A1/SERCA1 is key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction[1].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Sarcoplasmic reticulum membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Skeletal muscle, fast-twitch fibers (Type II)

    Induction:Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression

  • Isoforms & Post-Translational Modification

    O14983 has 3 isomers: O14983-1: 110252 Da (predicted); O14983-2: 109283 Da (predicted); O14983-3: 95199 Da (predicted).

  • Subunit

    Interacts with sarcolipin (SLN) (By similarity). Interacts with phospholamban (PLN) (By similarity). Interacts with myoregulin (MRLN). Interacts with DWORF (By similarity). Interacts with VMP1 (PubMed:28890335)

  • SwissProt ID

    O14983

  • Gene ID
    487 [NCBI]
  • Synonyms

    ATP2A; SERCA1

ATP2A1/SERCA1 Antibody (YA3519) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ATP2A1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ATP2A1/SERCA1 Antibody (YA3519) Product Image
    1/16
  • Experimental Validation Results for ATP2A1/SERCA1 Antibody (YA3519)
  • ATP2A1/SERCA1 Antibody (YA3519) Product Image
  • Experimental Validation Results for ATP2A1/SERCA1 Antibody (YA3519)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling ATP2A1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ATP2A1/SERCA1 Antibody (YA3519)
  • ATP2A1/SERCA1 Antibody (YA3519) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling ATP2A1 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ATP2A1/SERCA1 Antibody (YA3519) Product Image FC