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AKR1C1/AKR1C2 Antibody (YA2675)

(Synonyms: AKR1C1; AKR1C2)
Customer Review

Based on 1 Customer Validation

AKR1C1/AKR1C2 Antibody (YA2675) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKR1C1/AKR1C2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

AKR1C1/AKR1C2 Antibody (YA2675) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKR1C1/AKR1C2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 37 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 37 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human AKR1C1 / AKR1C2 aa50-101/323.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105049

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
    Western blot analysis of extracts from Hela(lane 2(20ug)，HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using AKR1C1/AKR1C2 Antibody(HY-P82930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
    Flow cytometric analysis of 1X10^6 HepG2 cells labeling AKR1C1/AKR1C2 Antibody (YA2675) (HY-P82930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    AKR1C1/AKR1C2 is a Q04828: Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids
    P52895: Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH. Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens. Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha-DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Also specifically able to produce 17beta-hydroxy-5alpha-androstan-3-one/5alphaDHT. May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate. Displays affinity for bile acids
    [1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Q04828: Cytoplasm, cytosol
    P52895: Cytoplasm, cytosol

  • Expression


    Tissue_specificity: Q04828: Expressed in all tissues tested including liver, prostate, testis, adrenal gland, brain, uterus, mammary gland and keratinocytes. Highest levels found in liver, mammary gland and brain
    P52895: Expressed in fetal testes. Expressed in fetal and adult adrenal glands

  • Isoforms & Post-Translational Modification

    P52895 has 2 isomers: P52895-1: 36735 Da (predicted); P52895-2: 15748 Da (predicted).

  • Subunit

    Q04828: Monomer

  • SwissProt ID

    Q04828 / P52895

  • Gene ID
    1645 [NCBI] / 1646 [NCBI]
  • Synonyms

    AKR1C1; AKR1C2

  • Research Field

    Signal Transduction

AKR1C1/AKR1C2 Antibody (YA2675) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using AKR1C1/AKR1C2 Antibody(HY-P82930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling AKR1C1/AKR1C2 Antibody (YA2675) (HY-P82930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image
    1/16
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug)，HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using AKR1C1/AKR1C2 Antibody(HY-P82930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 HepG2 cells labeling AKR1C1/AKR1C2 Antibody (YA2675) (HY-P82930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • Experimental Validation Results for AKR1C1/AKR1C2 Antibody (YA2675)
  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug)，HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using AKR1C1/AKR1C2 Antibody(HY-P82930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 HepG2 cells labeling AKR1C1/AKR1C2 Antibody (YA2675) (HY-P82930, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image WB
  • AKR1C1/AKR1C2 Antibody (YA2675) Product Image FC