Dopamine Transporter Antibody (YA6548)

Cat. No.: HY-P86855: Data Sheet User Guide for Antibodies
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Dopamine Transporter Antibody (YA6548) is a Rat -derived and non-conjugated monoclonal antibody, targeting to Dopamine Transporter.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Dopamine Transporter Antibody (YA6548) is a Rat -derived and non-conjugated monoclonal antibody, targeting to Dopamine Transporter.

Host

Rat

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 69 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse, Rat Predicted Reactivity: Human,Cynomolgus monkey,Pig

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q61327

Gene ID

13162 [NCBI]

Immunogen

Recombinant protein.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-F IHC-F: Immunohistochemistry-Frozen	1:500
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000
WB WB: Western Blot	1:2000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Solution

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Mediates sodium- and chloride-dependent transport of dopamine (PubMed:10375632, PubMed:12606774). Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (PubMed:30936473)

Subcellular Localization：Cell membrane,Cell projection, neuron projection,Cell projection, axon

Expression：
Tissue_Specificity: Found in the substantia nigra and ventral tegmental dopamine neurons, in fibers of the medial forebrain bundle ascending into the striatum, and within dense fiber networks and varicosities in the dorsal and ventral striatum (at protein level) (PubMed:17296554, PubMed:19357284). Lower expression in the cortex (at protein level) (PubMed:19357284). Absent from the corpus callosum (PubMed:19357284). Expressed throughout the retina at postnatal day 8 (PubMed:30936473)

Isoforms & Post-Translational Modification：Q61327: 619 amino acids, molecular weight 68805 Da.
Phosphorylation by EF-2 kinase completely inactivates EF-2; it requires prior phosphorylation by CDK2 at Ser-595 during mitotic prometaphase丨Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine (By similarity)丨(Microbial infection) Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A, thus arresting protein synthesis丨ISGylated丨Proteolytically processed at two sites following phosphorylation by CSK丨SUMOylated following phosphorylation by CSK, promotes proteolytic cleavage

Subunit：Monomer (By similarity)

Synonyms

DA transporter antibody; DAT 1 antibody; DAT antibody; DAT1 antibody; Dopamine transporter 1 antibody; Dopamine transporter antibody; PKDYS antibody; SC6A3_HUMAN antibody; SLC6A3 antibody; Sodium dependent dopamine transporter antibody; DA transporter antibody; DAT 1 antibody; DAT antibody; DAT1 antibody; Dopamine transporter 1 antibody; Dopamine transporter antibody; PKDYS antibody; SC6A3_HUMAN antibody; SLC6A3 antibody; Sodium dependent dopamine transporter antibody; Sodium-dependent dopamine transporter antibody; Solute carrier family 6 (neurotransmitter transporter dopamine), member 3 antibody; Solute carrier family 6 (neurotransmitter transporter), member 3 antibody; Solute carrier family 6 member 3 antibody; Variable number tandem repeat (VNTR) antibody;

Documentation

User Guide for Antibodies (1077 KB)