Phospho-Tau (T231) Antibody (YA3477)

Cat. No.: HY-P83779: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Phospho-Tau (T231) Antibody (YA3477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (T231).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Phospho-Tau (T231) Antibody (YA3477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (T231).

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 35-70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat Predicted Reactivity: Pig

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P10636-8

Gene ID

4137 [NCBI]

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr231 of human Tau-F (P10636-8).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200-1:500

Purity	affinity purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

IHC-P

Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity (PubMed:21985311). The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both (PubMed:21985311, PubMed:32961270). Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isofo

Subcellular Localization：Cytoplasm, cytosol; Cell membrane; Peripheral membrane protein; Cytoplasm, cytoskeleton; Cell projection, axon; Cell projection, dendrite; Secreted

Expression：
Tissue specificity:
Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

Isoforms & Post-Translational Modification：Human (P10636): 9 isomers: PNS-tau, Tau-G: 79-80 kDa (predicted); Fetal-tau, Tau-A, Tau-B, Tau-C, Tau-D, Tau-E, Tau-F: 32-45 kDa (predicted).
Mouse (P10637): 6 isomers: PNS-tau: 76 kDa (predicted); Tau-A, Tau-B, Tau-C, Tau-D, Tau-E: 35-44 kDa (predicted).
Rat (P19332): 8 isomers: Tau-A, Tau-B, Tau-H: 71-81 kDa (predicted); Tau-C, Tau-D, Tau-E, Tau-F, Tau-G: 38-51 kDa (predicted).
Multiple serine and threonine residues are phosphorylated.
Polyubiquitinated.
O-glycosylated.

Subunit：Interacts with MARK1, MARK2, MARK3 and MARK4 (PubMed:23666762). Interacts with PSMC2 through SQSTM1 (By similarity). Interacts with SQSTM1 when polyubiquitinated (PubMed:15953362). Interacts with FKBP4 (By similarity). Binds to CSNK1D (PubMed:14761950). Interacts with SGK1 (PubMed:16982696). Interacts with EPM2A; the interaction dephosphorylates MAPT at Ser-396 (PubMed:19542233). Interacts with PIN1 (PubMed:11313338). Interacts with LRRK2 (PubMed:26014385). Interacts with LRP1, leading to endocy

RRID

AB_3718762

Synonyms

MAPT; MAPTL; MTBT1; TAU; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)