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Phospho-Tau (T231) Antibody (YA3477)

(Synonyms: MAPT; MAPTL; MTBT1; TAU; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau)
Customer Review

Based on 1 Customer Validation

Phospho-Tau (T231) Antibody (YA3477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (T231).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, IP, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Phospho-Tau (T231) Antibody (YA3477) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:2000 1:1000 1:200 1:200-1:500

Product Details

Description

Phospho-Tau (T231) Antibody (YA3477) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-Tau (T231).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 35-70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr231 of human Tau-F (P10636-8).

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718762

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Tau promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isofo[1][2].

  • Subcellular Localization

    Cytoplasm, cytosol; Cell membrane; Peripheral membrane protein; Cytoplasm, cytoskeleton; Cell projection, axon; Cell projection, dendrite; Secreted

  • Expression


    Tissue specificity:
    Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.

  • Isoforms & Post-Translational Modification

    Human (P10636): 9 isomers: PNS-tau, Tau-G: 79-80 kDa (predicted); Fetal-tau, Tau-A, Tau-B, Tau-C, Tau-D, Tau-E, Tau-F: 32-45 kDa (predicted).
    Mouse (P10637): 6 isomers: PNS-tau: 76 kDa (predicted); Tau-A, Tau-B, Tau-C, Tau-D, Tau-E: 35-44 kDa (predicted).
    Rat (P19332): 8 isomers: Tau-A, Tau-B, Tau-H: 71-81 kDa (predicted); Tau-C, Tau-D, Tau-E, Tau-F, Tau-G: 38-51 kDa (predicted).
    Multiple serine and threonine residues are phosphorylated.
    Polyubiquitinated.
    O-glycosylated.

  • Subunit

    Interacts with MARK1, MARK2, MARK3 and MARK4 (PubMed:23666762). Interacts with PSMC2 through SQSTM1 (By similarity). Interacts with SQSTM1 when polyubiquitinated (PubMed:15953362). Interacts with FKBP4 (By similarity). Binds to CSNK1D (PubMed:14761950). Interacts with SGK1 (PubMed:16982696). Interacts with EPM2A; the interaction dephosphorylates MAPT at Ser-396 (PubMed:19542233). Interacts with PIN1 (PubMed:11313338). Interacts with LRRK2 (PubMed:26014385). Interacts with LRP1, leading to endocy

  • SwissProt ID

    P10636-8

  • Gene ID
    4137 [NCBI]
  • Synonyms

    MAPT; MAPTL; MTBT1; TAU; Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau

Phospho-Tau (T231) Antibody (YA3477) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Tau (T231) Antibody (YA3477) Product Image
    1/16
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Phospho-Tau (T231) Antibody (YA3477) Product Image
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Experimental Validation Results for Phospho-Tau (T231) Antibody (YA3477)
  • Phospho-Tau (T231) Antibody (YA3477) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Tau (T231) Antibody (YA3477) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using Phospho-Tau Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Phospho-Tau (T231) Antibody (YA3477) Product Image IHC-P
  • Phospho-Tau (T231) Antibody (YA3477) Product Image IHC-P