Cat. No.: HY-P81425

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S100 A8 Antibody (YA1170)

Name: S100 A8 Antibody (YA1170)
Brand: MedChemExpress
SKU: HY-P81425
Price: 84.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: Calgranulin-A antibody; Calprotectin L1L subunit antibody; CFAG antibody; Cystic fibrosis antigen antibody; Leukocyte L1 complex light chain antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; N-terminally processed antibody; p8 antibody; Protein S100-A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody)

Customer Review

Based on 1 Customer Validation

S100 A8 Antibody (YA1170) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 A8.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, IHC-P, ICC/IF, IP, FC, IF-Tissue
Reactivity :
Human

Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Conjugation:
Non-conjugated

ALL WB IHC-P FC

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Price

Stock

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Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Verification Images
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot	IHC-P IHC-P: Immunohistochemistry-Paraffin	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	FC FC: Flow Cytometry	IF-Tissue IF-Tissue: Immunofluorescence-Tissue	IP IP: Immunoprecipitation
Dilution Ratio	1:2000-1:5000	1:10000	1:50000	1:1000	1:1000-1:3000	1-2μg/sample

Product Details

Description

S100 A8 Antibody (YA1170) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 A8.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 14 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 11 kDa

Species Reactivity Database

Entrez Gene: 6279 Human

SwissProt: P05109 Human

OMIM: 123885 Human

Immunogen

Recombinant protein within human S100A8 aa 1-93/93.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103540

Product Properties

Appearance

Solution
Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Verification Images

Western blot analysis of extracts from SK-BR-3(lane 2(20ug) and SK-BR-3(lane 3(40ug) using S100 A8 Antibody (HY-P81425) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human oral cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human throat cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human skin tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human spleen tissue using S100 A8 Antibody (HY-P81425, 1/10000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 1X10⁶ THP-1 cells labeling S100 A8 Antibody (HY-P81425, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Flow cytometric analysis of 1X10⁶ THP-1 cells labeling S100 A8 Antibody (HY-P81425, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/1000 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function

S100A8 is a calcium- and zinc-binding member of the S100 protein family that plays a prominent role in regulating inflammatory processes and innate immune responses, and it is most commonly present as the calprotectin complex together with S100A9^[4]. Mechanistically, S100A8 functions as a damage-associated molecular pattern (DAMP) and participates in activation of TLR4- and AGER-dependent signaling, leading to downstream NF-κB and MAPK pathway activation that amplifies inflammatory responses. Through these pathways, S100A8 contributes to neutrophil chemotaxis, leukocyte adhesion, antimicrobial defense, and coordination of myeloid-cell-driven inflammation^[4]. In disease settings, elevated S100A8/S100A9 signaling has been associated with inflammatory disorders, cardiovascular pathology, and tissue injury responses, supporting its value as a biomarker and mechanistic mediator of disease progression^[1]^[2]^[3]. Experimental studies and clinical observations further indicate that circulating S100A8/A9 levels can provide prognostic information in cardiovascular disease, including heart failure following acute myocardial infarction^[3]. Compared with the related isoform S100A9, S100A8 is frequently studied as a component of the S100A8/S100A9 heterodimer rather than as an isolated protein, reflecting the dominant biological activity of calprotectin in inflammatory microenvironments^[4]. For experimental applications, inhibition of S100A8/A9 activity using small-molecule inhibitors or antibodies has improved pathological outcomes in murine models, making this signaling axis a useful target for mechanistic and translational research^[2].
Subcellular Localization

Secreted; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Peripheral membrane protein
Expression

Tissue_specificity:Calprotectin (S100A8/9) is primarily expressed in myeloid cells. Except in inflammatory states, its expression is limited to specific stages of myeloid differentiation, as both proteins are expressed in circulating neutrophils and monocytes, but absent in normal tissue macrophages and lymphocytes. Calprotectin is also expressed in the epidermis in cH2O2ic inflammatory states, such as psoriasis and malignancies. Calprotectin concentrations are higher in serum at sites of inflammation or in patients with inflammatory diseases such as rheumatoid arthritis, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, Sjgren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Calprotectin is involved in the formation and deposition of amyloid proteins (called amyloid inclusion bodies) in the aging prostate. It is significantly upregulated in a variety of tumors, including gastric cancer, esophageal cancer, colon cancer, pancreatic cancer, bladder cancer, ovarian cancer, thyroid cancer, breast cancer, and skin cancer.

Induction: (Microbial infection) Expression is highly induced in CD14 (+) monocytes, neutrophils, and developing neutrophils of infected by SARS-COV-2

Positive sample: SK-Br-3 cell lysate, human breast carcinoma tissue, human liver tissue, human spleen tissue, human tonsil tissue, SK-Br-3.
Subunit

Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A9 known as calprotectin (S100A8/A9).
SwissProt ID

P05109
Gene ID

6279 [NCBI]
Synonyms

Calgranulin-A antibody; Calprotectin L1L subunit antibody; CFAG antibody; Cystic fibrosis antigen antibody; Leukocyte L1 complex light chain antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; N-terminally processed antibody; p8 antibody; Protein S100-A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody
Research Field

Immunology