S100 A8 Antibody (YA1170)

Cat. No.: HY-P81425: Data Sheet COA
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S100 A8 Antibody (YA1170) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 A8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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S100 A8 Antibody (YA1170) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

S100 A8 Antibody (YA1170) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100 A8.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: 14 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P05109

Gene ID

6279 [NCBI]

Immunogen

Recombinant protein within human S100A8 aa 1-93/93.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000-1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50000
FC FC: Flow Cytometry	1:1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:1000-1:3000
IP IP: Immunoprecipitation	1-2μg/sample

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from SK-BR-3(lane 2(20ug) and SK-BR-3(lane 3(40ug) using S100 A8 Antibody (HY-P81425) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH2O2idase. Also participates in regulatory T-cell differentiation together with CD69 (PubMed:26296369). Activates NADPH2O2idase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its pro-inflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the pro-inflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs tH2O2gh the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity; (Microbial infection) Upon infection by human coronavirus SARS-CoV-2, may induce expansion of aberrant immature neutrophils in a TLR4-dependent manner

Subcellular Localization：Secreted; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Peripheral membrane protein

Expression：
Tissue_specificity:Calprotectin (S100A8/9) is primarily expressed in myeloid cells. Except in inflammatory states, its expression is limited to specific stages of myeloid differentiation, as both proteins are expressed in circulating neutrophils and monocytes, but absent in normal tissue macrophages and lymphocytes. Calprotectin is also expressed in the epidermis in cH2O2ic inflammatory states, such as psoriasis and malignancies. Calprotectin concentrations are higher in serum at sites of inflammation or in patients with inflammatory diseases such as rheumatoid arthritis, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, Sjgren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Calprotectin is involved in the formation and deposition of amyloid proteins (called amyloid inclusion bodies) in the aging prostate. It is significantly upregulated in a variety of tumors, including gastric cancer, esophageal cancer, colon cancer, pancreatic cancer, bladder cancer, ovarian cancer, thyroid cancer, breast cancer, and skin cancer.

Induction: (Microbial infection) Expression is highly induced in CD14 (+) monocytes, neutrophils, and developing neutrophils of infected by SARS-COV-2

Positive sample: SK-Br-3 cell lysate, human breast carcinoma tissue, human liver tissue, human spleen tissue, human tonsil tissue, SK-Br-3.

Subunit：Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A9 known as calprotectin (S100A8/A9).

RRID

AB_3103540

Database

Entrez Gene: 6279 Human

SwissProt: P05109 Human

OMIM: 123885 Human

Research Field

Immunology

Synonyms

Calgranulin-A antibody; Calprotectin L1L subunit antibody; CFAG antibody; Cystic fibrosis antigen antibody; Leukocyte L1 complex light chain antibody; Migration inhibitory factor-related protein 8 antibody; MRP-8 antibody; N-terminally processed antibody; p8 antibody; Protein S100-A8 antibody; S100 calcium-binding protein A8 antibody; S100A8 antibody; S10A8_HUMAN antibody; Urinary stone protein band A antibody

Documentation

Select Batch:

Data Sheet (236 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

S100 A8 Antibody (YA1170) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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S100 A8 Antibody (YA1170)

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