CD33 Antibody (YA3860)(PBS only)
(Synonyms: p67; SIGLEC3; SIGLEC-3)CD33 Antibody (YA3860) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD33.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
CD33 Antibody (YA3860) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD33.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 67 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 67 kDa
Purified recombinant fragment of human CD33 (AA: 15-237) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD33 is a Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase/PI3K[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Peroxisome
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Expression
Tissue_specificity:Monocytic/myeloid lineage cells. In the brain, CD33 is mainly expressed on microglial cells -
Isoforms & Post-Translational Modification
P20138 has 3 isomers: P20138-1: 39825 Da (predicted); P20138-2: 33890 Da (predicted); P20138-3: 25293 Da (predicted).
Glycosylated. Glycosylation at Asn-100 is critical for regulating ligand recognition;Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6. LCK phosphorylates Tyr-340 efficiently and Tyr-358 to a lesser extent -
Subunit
Homodimer; disulfide-linked (PubMed:10887109). Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation (PubMed:10206955, PubMed:10556798).
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SwissProt ID
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Synonyms
p67; SIGLEC3; SIGLEC-3
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