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IGF1 Antibody

(Synonyms: IBP1; IGF 1; IGF I; IGF-1; IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Full=Insulin-like growth factor I; Insulin Like Growth Factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; MGF; Mechano growth factor; MGF; Somatomedia C; Somatomedin C; IGF1_HUMAN. IGF-I; )
Customer Review

Based on 1 Customer Validation

IGF1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IGF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse, Rat, Dog

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02 or 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • IGF1 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:20-100 1:100-500 1:100-500 1:100-500

Product Details

Description

IGF1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to IGF1.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat, Dog Predicted Reactivity: Pig,Cow,Rabbit,Sheep
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 7.7/21kD Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 7.7/21kDa；
Species Reactivity Database

Entrez Gene: 3479 Human

SwissProt: P05019 Human

Immunogen

KLH conjugated synthetic peptide derived from human IGF-1: 49-100/195

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103063

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02 or 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGF1 Antibody
    Western blot analysis of extracts from M-Liver using IGF1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for IGF1 Antibody
    Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF1 Antibody
    Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    IGF1 is a The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM[1][2][3][4][5][6].

  • Subcellular Localization

    Secreted

  • Isoforms & Post-Translational Modification

    P05019 has 4 isomers: P05019-1: 21841 Da (predicted); P05019-2: 17026 Da (predicted); P05019-3: 15177 Da (predicted); P05019-4: 17762 Da (predicted).

  • Subunit

    Forms a ternary complex with IGFR1 and ITGAV:ITGB3 (PubMed:19578119). Forms a ternary complex with IGFR1 and ITGA6:ITGB4 (PubMed:22351760, PubMed:23696648). Interacts with SH2D3C isoform 2 (PubMed:20881139). Forms a ternary complex with IGFBP3 and ALS (PubMed:35907924)

  • SwissProt ID

    P05019

  • Gene ID
    3479 [NCBI]
  • Synonyms

    IBP1; IGF 1; IGF I; IGF-1; IGF IA; IGF IB; IGF1; IGFI; IGFIA; Insulin like growth factor 1 (somatomedin C); Full=Insulin-like growth factor I; Insulin Like Growth Factor 1; Insulin like growth factor IA; Insulin like growth factor IB; Mechano growth factor; MGF; Mechano growth factor; MGF; Somatomedia C; Somatomedin C; IGF1_HUMAN. IGF-I;

[1]. Zoidis E, et al. Stimulation of glucose transport in osteoblastic cells by parathyroid hormone and insulin-like growth factor I. Mol Cell Biochem. 2011 Feb;348(1-2):33-42. [Content Brief]

[2]. Shcheglovitov A, et al. SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients. Nature. 2013 Nov 14;503(7475):267-71. [Content Brief]

[3]. Saegusa J, et al. The direct binding of insulin-like growth factor-1 (IGF-1) to integrin alphavbeta3 is involved in IGF-1 signaling. J Biol Chem. 2009 Sep 4;284(36):24106-14. [Content Brief]

[4]. Fujita M, et al. Cross-talk between integrin α6β4 and insulin-like growth factor-1 receptor (IGF1R) through direct α6β4 binding to IGF1 and subsequent α6β4-IGF1-IGF1R ternary complex formation in anchorage-independent conditions. J Biol Chem. 2012 Apr 6;287(15):12491-500. [Content Brief]

[5]. Fujita M, et al. Insulin-like growth factor (IGF) signaling requires αvβ3-IGF1-IGF type 1 receptor (IGF1R) ternary complex formation in anchorage independence, and the complex formation does not require IGF1R and Src activation. J Biol Chem. 2013 Feb 1;288(5):3059-69. [Content Brief]

[6]. Fujita M, et al. An integrin binding-defective mutant of insulin-like growth factor-1 (R36E/R37E IGF1) acts as a dominant-negative antagonist of the IGF1 receptor (IGF1R) and suppresses tumorigenesis but still binds to IGF1R. J Biol Chem. 2013 Jul 5;288(27):19593-603. [Content Brief]

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IGF1 Antibody Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from M-Liver using IGF1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF1 Antibody Product Image
    1/16
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • IGF1 Antibody Product Image
  • Experimental Validation Results for IGF1 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from M-Liver using IGF1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for IGF1 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF1 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • Experimental Validation Results for IGF1 Antibody
  • IGF1 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-Liver using IGF1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • IGF1 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF1 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling IGF1 with IGF1 Antibody (HY-P81112) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with IGF1 Antibody (HY-P81112) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • IGF1 Antibody Product Image WB
  • IGF1 Antibody Product Image ICC
  • IGF1 Antibody Product Image ICC