IGFBP2 Antibody (YA1589)

Cat. No.: HY-P81844: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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IGFBP2 Antibody (YA1589) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P18065

Gene ID

3485 [NCBI]

Immunogen

A synthesized peptide derived from human IGFBP2 aa280-325/325.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18563800, PubMed:38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed:22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed:16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed:26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed:38796567)

Subcellular Localization：Secreted

Subunit：Interacts with IGF1 (PubMed:19081843). Interacts with IGF2 (PubMed:1726837, PubMed:18563800). Interacts (via RGD motif) with integrin alpha5/ITGA5; this interaction induces cell migration, adhesion or apoptosis according to the context (PubMed:16569642, PubMed:26076738, PubMed:38796567). Interacts with PTPRB; this interaction leads to PTPRB dimerization and inactivation (PubMed:22869525)

RRID

AB_3103943

Database

Entrez Gene: 3485 Human

SwissProt: P18065 Human

OMIM: 146731 Human

Research Field

Signal Transduction

Synonyms

IGFBP2; BP2; IBP2; Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

IGFBP2 Antibody (YA1589) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IGFBP2 Antibody (YA1589)

IGFBP2 Antibody (YA1589) Featured Recommendations:

Product Detail

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Background

Documentation

IGFBP2 Antibody (YA1589) Related Classifications

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