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IGFBP2 Antibody (YA1589)

(Synonyms: IGFBP2; BP2; IBP2; Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2)
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Based on 1 Customer Validation

IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IGFBP2 Antibody (YA1589)
  • IGFBP2 Antibody (YA1589) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 35 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 35 kDa
Species Reactivity Database

Entrez Gene: 3485 Human

SwissProt: P18065 Human

Immunogen

A synthesized peptide derived from human IGFBP2 aa280-325/325.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103943

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IGFBP2 Antibody (YA1589)
    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    IGFBP2 multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner. Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation. Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif. Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells. Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury[1][2][3][4][5].

  • Subcellular Localization

    Secreted

  • Subunit

    Interacts with IGF1 (PubMed:19081843). Interacts with IGF2 (PubMed:1726837, PubMed:18563800). Interacts (via RGD motif) with integrin alpha5/ITGA5; this interaction induces cell migration, adhesion or apoptosis according to the context (PubMed:16569642, PubMed:26076738, PubMed:38796567). Interacts with PTPRB; this interaction leads to PTPRB dimerization and inactivation (PubMed:22869525)

  • SwissProt ID

    P18065

  • Gene ID
    3485 [NCBI]
  • Synonyms

    IGFBP2; BP2; IBP2; Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2

  • Research Field

    Signal Transduction

IGFBP2 Antibody (YA1589) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGFBP2 Antibody (YA1589) Product Image
    1/16
  • Experimental Validation Results for IGFBP2 Antibody (YA1589)
  • IGFBP2 Antibody (YA1589) Product Image
  • Experimental Validation Results for IGFBP2 Antibody (YA1589)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IGFBP2 Antibody (YA1589)
  • IGFBP2 Antibody (YA1589) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IGFBP2 Antibody (YA1589) Product Image WB