IGFBP2 Antibody (YA1589)

製品番号: HY-P81844: データシート SDS COA User Guide for Antibodies
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IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

IGFBP2 Antibody (YA1589) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IGFBP2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 35 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P18065

Gene ID

3485 [NCBI]

Immunogen

A synthesized peptide derived from human IGFBP2 aa280-325/325.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from SH-SY5Y (lane 1, 20 μg), A549 (lane 2, 20 μg), and Raw264.7 (lane 3, 20 μg) using IGFBP2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:18563800, PubMed:38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed:22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed:16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed:26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed:38796567)

Subcellular Localization：Secreted

Subunit：Interacts with IGF1 (PubMed:19081843). Interacts with IGF2 (PubMed:1726837, PubMed:18563800). Interacts (via RGD motif) with integrin alpha5/ITGA5; this interaction induces cell migration, adhesion or apoptosis according to the context (PubMed:16569642, PubMed:26076738, PubMed:38796567). Interacts with PTPRB; this interaction leads to PTPRB dimerization and inactivation (PubMed:22869525)

RRID

AB_3103943

Database

Entrez Gene: 3485 Human

SwissProt: P18065 Human

OMIM: 146731 Human

Research Field

Signal Transduction

Synonyms

IGFBP2; BP2; IBP2; Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2

ドキュメンテーション

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データシート (258 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)