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Septin 7 Antibody (YA1927)

(Synonyms: septin 7; CDC3; CDC10; SEPT7; SEPT7A; NBLA02942)
Customer Review

Based on 1 Customer Validation

Septin 7 Antibody (YA1927) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Septin 7.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Septin 7 Antibody (YA1927) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000-1:5000 1:50-1:100

Product Details

Description

Septin 7 Antibody (YA1927) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Septin 7.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 51 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 51 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human SEPT7 aa286-435.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), U251 (lane 4(20μg), Raji (lane 5(20μg) using Septin 7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Septin 7 is a Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation[1].

  • Subcellular Localization

    Cytoplasm; Chromosome, centromere, kinetochore; Cytoplasm, cytoskeleton, spindle; Cleavage furrow; Midbody; Cytoplasm, cytoskeleton, cilium axoneme; Cell projection, cilium, flagellum

  • Expression


    Tissue_specificity:Widely expressed

  • Isoforms & Post-Translational Modification

    Q16181 has 2 isomers: Q16181-1: 50680 Da (predicted); Q16181-2: 50581 Da (predicted).

  • Subunit

    Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation.

  • SwissProt ID

    Q16181

  • Gene ID
    989 [NCBI]
  • Synonyms

    septin 7; CDC3; CDC10; SEPT7; SEPT7A; NBLA02942

  • Research Field

    Tags & Cell Markers

Septin 7 Antibody (YA1927) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), U251 (lane 4(20μg), Raji (lane 5(20μg) using Septin 7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Septin 7 Antibody (YA1927) Product Image
    1/16
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Septin 7 Antibody (YA1927) Product Image
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), U251 (lane 4(20μg), Raji (lane 5(20μg) using Septin 7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Experimental Validation Results for Septin 7 Antibody (YA1927)
  • Septin 7 Antibody (YA1927) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg), C6 (lane 3(20μg), U251 (lane 4(20μg), Raji (lane 5(20μg) using Septin 7 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Septin 7 Antibody (YA1927) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Septin 7 Antibody (YA1927) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Septin 7 Antibody with Septin 7 Antibody (YA1927) (HY-P82182) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Septin 7 Antibody (YA1927) ((HY-P82182) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Septin 7 Antibody (YA1927) Product Image WB
  • Septin 7 Antibody (YA1927) Product Image ICC
  • Septin 7 Antibody (YA1927) Product Image ICC