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USP11 Antibody (YA1846)

(Synonyms: UHX1; USP11)
Customer Review

Based on 1 Customer Validation

USP11 Antibody (YA1846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP11.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • USP11 Antibody (YA1846) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:200-1:1000 1:10-1:100

Product Details

Description

USP11 Antibody (YA1846) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP11.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human USP11

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Liquid in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for USP11 Antibody (YA1846)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), 293T (lane 3, 15 μg), IMR-32 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using USP11 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for USP11 Antibody (YA1846)
    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using USP11 antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    USP11 (ubiquitin-specific peptidase 11) is an X-linked deubiquitinating enzyme of the ubiquitin-specific protease family that primarily regulates protein stability by removing ubiquitin chains from substrate proteins and thereby modulating protein turnover and signaling networks[1]. Mechanistically, USP11 functions in chromatin regulation and the cellular DNA damage response, where it acts as a histone deubiquitinase that contributes to chromatin reorganization during DNA repair processes[2]. Through these activities, USP11 participates in pathways controlling DNA double-strand break repair, cell-cycle progression, and signal transduction, highlighting its importance in maintaining genome integrity[3]. In disease contexts, dysregulated USP11 activity has been linked to multiple cancer models, where stabilization of specific substrates can influence tumor growth, survival, and therapeutic responses[1][3]. Compared with its closest paralogs, USP4 and USP15, USP11 belongs to the same DUSP-UBL domain-containing subfamily but exhibits distinct regulatory mechanisms and substrate specificity despite overall structural similarity, making isoform-selective investigation particularly important for mechanistic studies[3]. This functional divergence has increased interest in USP11 as a research target for understanding deubiquitinase-dependent signaling pathways and disease biology[1][3]. For experimental applications, peptide ligands that recognize a specific ubiquitin-like domain-binding site of USP11 have been developed, providing tools to interrogate USP11-specific molecular interactions[4]. More recently, selective USP11 inhibitors have been reported, enabling pharmacological disruption of USP11-dependent signaling and supporting target-validation studies in cancer research models[5].

  • Subcellular Localization

    Nucleus; Cytoplasm; Chromosome

  • Subunit

    Monomer (PubMed:24724799). Associated component of the Polycomb group (PcG) multiprotein PRC1-like complex (PubMed:20601937). Interacts with RANBP9/RANBPM (PubMed:12084015). Interacts with BRCA2 (PubMed:15314155). Interacts with CHUK/IKKA (PubMed:17897950). Interacts with NFKBIA (PubMed:19874889). Interacts with SPRY3, RAE1, MYCBP2/PAM, and KCTD6 (PubMed:29293652)

  • SwissProt ID

    P51784

  • Gene ID
    8237 [NCBI]
  • Synonyms

    UHX1; USP11

  • Research Field

    Epigenetics and Nuclear Signaling

References

[1]. Guo T, et al. The Dual Role of USP11 in Cancer. J Oncol. 2022 Mar 22;2022:9963905. [Content Brief]

[2]. Ting X, et al. USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair. Nucleic Acids Res. 2019 Oct 10;47(18):9721-9740. [Content Brief]

[3]. Maurer SK, et al. Ubiquitin-specific protease 11 structure in complex with an engineered substrate mimetic reveals a molecular feature for deubiquitination selectivity. J Biol Chem. 2023 Nov;299(11):105300. [Content Brief]

[4]. Spiliotopoulos A, et al. Discovery of peptide ligands targeting a specific ubiquitin-like domain-binding site in the deubiquitinase USP11. J Biol Chem. 2019 Jan 11;294(2):424-436. [Content Brief]

[5]. Kayastha F, et al. Discovery, development, and characterization of potent and selective USP11 inhibitors. Pharmacol Res. 2026 Jan;223:108075. [Content Brief]

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USP11 Antibody (YA1846) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), 293T (lane 3, 15 μg), IMR-32 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using USP11 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using USP11 antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • USP11 Antibody (YA1846) Product Image
    1/16
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • USP11 Antibody (YA1846) Product Image
  • Experimental Validation Results for USP11 Antibody (YA1846)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), 293T (lane 3, 15 μg), IMR-32 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using USP11 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for USP11 Antibody (YA1846)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using USP11 antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • Experimental Validation Results for USP11 Antibody (YA1846)
  • USP11 Antibody (YA1846) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 15 μg), Hela (lane 2, 15 μg), 293T (lane 3, 15 μg), IMR-32 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using USP11 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin(HRP), HY-P80955A, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • USP11 Antibody (YA1846) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 1), SH-SY5Y (lane 2), HeLa (lane 3), HepG2 (lane 4), A549 (lane 5), and Caco-2 (lane 6) using USP11 antibody. Proteins were transferred onto a 0.45 μm NC membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • USP11 Antibody (YA1846) Product Image WB
  • USP11 Antibody (YA1846) Product Image WB