SLC31A1 Antibody (YA1408)(PBS only)

Cat. No.: HY-P81663A: Data Sheet User Guide for Antibodies
FAQs
Technical Support

SLC31A1 Antibody (YA1408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC31A1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SLC31A1 Antibody (YA1408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC31A1.

Host

Rabbit

Molecular Weight

Predicted band size: 21 kDa;

Observed band size: 26-34 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Hamster

SwissProt ID

O15431

Gene ID

100689094 Hamster 1317 [NCBI]

Immunogen

A synthetic peptide of human SLC31A1/CTR1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Uniporter that mediates the transport of copper(1+) from the extracellular space to the cytoplasm, across the plasma membrane (PubMed:11734551, PubMed:16135512, PubMed:17525160, PubMed:19740744, PubMed:20451502, PubMed:20569931, PubMed:23658018) and delivers directly copper(1+) to specific chaperone such as ATOX1, via a copper(1+)- mediated transient interaction between the C-terminal domain and a copper(1+) chaperone, thus controlling intracellular copper(1+) levels (PubMed:11734551, PubMed:16135512, PubMed:17525160, PubMed:19740744, PubMed:20451502, PubMed:20569931, PubMed:23658018, PubMed:26745413). May function in copper(1+) import from the apical membrane thus may drive intestinal copper absorption (By similarity). The copper(1+) transport mechanism is sodium-independent, saturable and of high-affinity (PubMed:11734551). Also mediates the uptake of silver(1+) (PubMed:20569931). May function in the influx of the platinum-containing chemotherapeutic agents (PubMed:20451502, PubMed:20569931). The platinum-containing chemotherapeutic agents uptake is saturable (By similarity). In vitro, mediates the transport of cadmium(2+) into cells (PubMed:33294387). Also participates in the first step of copper(2+) acquisition by cells through a direct transfer of copper(2+) from copper(2+) carriers in blood, such as ALB to the N-terminal domain of SLC31A1, leading to copper(2+) reduction and probably followed by copper(1+) stabilization (PubMed:30489586). In addition, functions as a redox sensor to promote angiogenesis in endothelial cells, in a copper(1+) transport independent manner, by transmitting the VEGF-induced ROS signal through a sulfenylation at Cys-189 leadin g to a subsequent disulfide bond formation between SLC31A1 and KDR (PubMed:35027734). The SLC31A1-KDR complex is then co-internalized to early endosomes, driving a sustained VEGFR2 signaling (PubMed:35027734); Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Early endosome membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

Subunit：Homotrimer (PubMed:11734551, PubMed:15326162, PubMed:16501047); is stabilized by cisplatin (PubMed:15326162) via interactions between cisplatin and the methionine-rich clusters(PubMed:15326162), and could be crucial for the copper(2+) reduction process and copper(1+) stabilization (PubMed:11734551, PubMed:15326162, PubMed:16501047, PubMed:32914794). Heterotrimer between SLC31A1, CCS and SOD1; this heterotrimer is copper(1+)-mediated and its maintenance is regulated through SOD1 activation (PubMed:31292775). Interacts with KDR; this interaction is induced upon VEGFA stimulation leading to SLC31A1 and KDR subsequent co-internalization to early endosomes, thereby activating KDR downstream signaling in endothelial cells (PubMed:35027734). Interacts (via C-terminal domain) with ATOX1 (via dimer form); this interaction improves ATOX1 stability and controls intracellular copper(1+) levels (PubMed:24837030, PubMed:26745413). Interacts with SLC31A2; this interaction stabilizes SLC31A2 and protects its from ubiquitination and degradation (PubMed:26205368). Interacts (via C-terminal domain) with CCS; this interaction is copper(1+)-mediated (PubMed:31292775)

Research Field

Neuroscience

Synonyms

SLC31A1; COPT1; CTR1; solute carrier family 31 member 1

Documentation

User Guide for Antibodies (1077 KB)