SLC31A1 Antibody (YA1408)
(Synonyms: SLC31A1; COPT1; CTR1; solute carrier family 31 member 1)
Based on 1 Customer Validation
SLC31A1 Antibody (YA1408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC31A1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Human, Mouse, Rat, Hamster
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
SLC31A1 Antibody (YA1408) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLC31A1.
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Host Rabbit
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Clonality Recombinant, Monoclonal
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Species ReactivityHuman, Mouse, Rat, Hamster
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Observed Molecular WeightObserved band size: 26-34 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 21 kDa
Entrez Gene: 100689094 Hamster 1317 ; 20529 Mouse ; 171135 Rat
SwissProt: O15431 Human ; Q8K211 Mouse ; Q9JK41 Rat
OMIM: 620306 Human
A synthetic peptide of human SLC31A1/CTR1
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A549(lane 2(20ug) , Neuro-2a(lane 3(20ug) and C6(lane 4(20ug) using SLC31A1 Antibody (HY-P81663) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Background
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Function
SLC31A1 is an Uniporter that mediates the transport of copper(1+) from the extracellular space to the cytoplasm, across the plasma membrane and delivers directly copper(1+) to specific chaperone such as ATOX1, via a copper(1+)- mediated transient interaction between the C-terminal domain and a copper(1+) chaperone, thus controlling intracellular copper(1+) levels. May function in copper(1+) import from the apical membrane thus may drive intestinal copper absorption. The copper(1+) transport mechanism is sodium-independent, saturable and of high-affinity. Also mediates the uptake of silver(1+). May function in the influx of the platinum-containing chemotherapeutic agents. The platinum-containing chemotherapeutic agents uptake is saturable. In vitro, mediates the transport of cadmium(2+) into cells. Also participates in the first step of copper(2+) acquisition by cells through a direct transfer of copper(2+) from copper(2+) carriers in blood, such as ALB to the N-terminal domain of SLC31A1, leading to copper(2+) reduction and probably followed by copper(1+) stabilization. In addition, functions as a redox sensor to promote angiogenesis in endothelial cells, in a copper(1+) transport independent manner, by transmitting the VEGF-induced ROS signal through a sulfenylation at Cys-189 leadin g to a subsequent disulfide bond formation between SLC31A1 and KDR. The SLC31A1-KDR complex is then co-internalized to early endosomes, driving a sustained VEGFR2 signaling; Mobilizes copper(1+) out of the endosomal compartment, making copper(1+) available for export out of the cells[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cell membrane; Multi-pass membrane protein; Early endosome membrane; Multi-pass membrane protein; Recycling endosome membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein; Late endosome membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein
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Subunit
Homotrimer (PubMed:11734551, PubMed:15326162, PubMed:16501047); is stabilized by cisplatin (PubMed:15326162) via interactions between cisplatin and the methionine-rich clusters(PubMed:15326162), and could be crucial for the copper(2+) reduction process and copper(1+) stabilization (PubMed:11734551, PubMed:15326162, PubMed:16501047, PubMed:32914794). Heterotrimer between SLC31A1, CCS and SOD1; this heterotrimer is copper(1+)-mediated and its maintenance is regulated through SOD1 activation (PubMed:31292775). Interacts with KDR; this interaction is induced upon VEGFA stimulation leading to SLC31A1 and KDR subsequent co-internalization to early endosomes, thereby activating KDR downstream signaling in endothelial cells (PubMed:35027734). Interacts (via C-terminal domain) with ATOX1 (via dimer form); this interaction improves ATOX1 stability and controls intracellular copper(1+) levels (PubMed:24837030, PubMed:26745413). Interacts with SLC31A2; this interaction stabilizes SLC31A2 and protects its from ubiquitination and degradation (PubMed:26205368). Interacts (via C-terminal domain) with CCS; this interaction is copper(1+)-mediated (PubMed:31292775)
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SwissProt ID
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Gene ID
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Synonyms
SLC31A1; COPT1; CTR1; solute carrier family 31 member 1
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Research Field
Neuroscience
Documentation
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Data Sheet (231 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
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