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Pyruvate Dehydrogenase E2 Antibody (YA912)

(Synonyms: DLAT; DLTA; E2; PBC; PDCE2; Pyruvate Dehydrogenase E2)
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Based on 1 publication(s) in Google Scholar

Pyruvate Dehydrogenase E2 Antibody (YA912) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

Pyruvate Dehydrogenase E2 Antibody (YA912) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 69 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 69 kDa
Species Reactivity Database
Immunogen

Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3103049

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    Western blot analysis of extracts from A549(lane 2(20μg), U251 (lane 3(20μg) and Hela(lane 4(20μg) using DLAT (HY-P81070) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P83341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P81070) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Pyruvate Dehydrogenase E2 is an As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety (Probable). The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable)

  • Subcellular Localization

    Mitochondrion matrix

  • Subunit

    Interacts with PDK2 and PDK3 (PubMed:15861126, PubMed:17532006, PubMed:17683942, PubMed:18387944). Interacts with SIRT4 (PubMed:25525879). Interacts with PDHB (PubMed:20160912)

  • SwissProt ID

    P10515

  • Gene ID
    1737 [NCBI]
  • Synonyms

    DLAT; DLTA; E2; PBC; PDCE2; Pyruvate Dehydrogenase E2

  • Research Field

    Signal Transduction

Pyruvate Dehydrogenase E2 Antibody (YA912) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg), U251 (lane 3(20μg) and Hela(lane 4(20μg) using DLAT (HY-P81070) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P83341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P81070) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
    1/16
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549(lane 2(20μg), U251 (lane 3(20μg) and Hela(lane 4(20μg) using DLAT (HY-P81070) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P83341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P81070) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Experimental Validation Results for Pyruvate Dehydrogenase E2 Antibody (YA912)
  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549(lane 2(20μg), U251 (lane 3(20μg) and Hela(lane 4(20μg) using DLAT (HY-P81070) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P83341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P81070) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image WB
  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image ICC
  • Pyruvate Dehydrogenase E2 Antibody (YA912) Product Image ICC