Pyruvate Dehydrogenase E2 Antibody (YA912)

Cat. No.: HY-P81070: Data Sheet COA
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User Guide for Antibodies
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Pyruvate Dehydrogenase E2 Antibody (YA912) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Pyruvate Dehydrogenase E2 Antibody (YA912) Featured Recommendations:

1 Publications Citing Use of MCE Pyruvate Dehydrogenase E2 Antibody (YA912)

•ACS Appl Nano Mater. 2025 Jan 17.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Pyruvate Dehydrogenase E2 Antibody (YA912) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Pyruvate Dehydrogenase E2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 69 kDa;

Observed band size: 69 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P10515

Gene ID

1737 [NCBI]

Immunogen

Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from A549(lane 2(20μg), U251 (lane 3(20μg) and Hela(lane 4(20μg) using DLAT (HY-P81070) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P83341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling DLAT with DLAT antibody (HY-P81070) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DLAT antibody (HY-P81070) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：As part of the pyruvate dehydrogenase complex, catalyzes the transfers of an acetyl group to a lipoic acid moiety (Probable). The pyruvate dehydrogenase complex, catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable)

Subcellular Localization：Mitochondrion matrix

Subunit：Interacts with PDK2 and PDK3 (PubMed:15861126, PubMed:17532006, PubMed:17683942, PubMed:18387944). Interacts with SIRT4 (PubMed:25525879). Interacts with PDHB (PubMed:20160912)

RRID

AB_3103049

Database

Entrez Gene: 1737 Human ; 235339 Mouse ;

SwissProt: P10515 Human ; Q8BMF4 Mouse ;

OMIM: 245348 Human

Research Field

Signal Transduction

Synonyms

DLAT; DLTA; E2; PBC; PDCE2; Pyruvate Dehydrogenase E2

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Pyruvate Dehydrogenase E2 Antibody (YA912) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Pyruvate Dehydrogenase E2 Antibody (YA912)

Pyruvate Dehydrogenase E2 Antibody (YA912) Featured Recommendations:

1 Publications Citing Use of MCE Pyruvate Dehydrogenase E2 Antibody (YA912)

Product Detail

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Background

Documentation

Pyruvate Dehydrogenase E2 Antibody (YA912) Related Classifications

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