ROCK1 Antibody (YA6230)
(Synonyms: ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK)
Based on 1 Customer Validation
ROCK1 Antibody (YA6230) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:1000-1:5000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
ROCK1 Antibody (YA6230) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ROCK1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 158 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 158 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ROCK1 is a RhoA effector serine/threonine kinase that regulates actomyosin cytoskeleton organization, smooth-muscle contraction, cell migration, and cell-shape control[1][2]. Mechanistically, ROCK1 promotes myosin light-chain phosphorylation and actomyosin contractility, and caspase-3 cleavage converts ROCK1 into an active apoptotic effector that drives membrane blebbing[3]. In vivo, ROCK1-deficient mice show failed eyelid and ventral body-wall closure, supporting its role in epithelial morphogenesis and actomyosin bundle assembly[4]. ROCK1 also regulates Beclin1-mediated autophagy, linking ROCK1 signaling to the balance between apoptosis and autophagy[8]. Compared with ROCK2, ROCK1 shows isoform-specific biology: ROCK1 destabilizes stress fibers through MLC2 phosphorylation and peripheral actomyosin contraction, whereas ROCK2 stabilizes actin through cofilin phosphorylation[5]. In glioblastoma models, ROCK1 is upregulated relative to normal brain tissue, and ROCK1 knockdown reduces proliferation and alters substrate-dependent migration, while ROCK2 knockdown produces different migration and proliferation effects[6]. For experimental applications, ROCK inhibitors provide tools to test ROCK-dependent contraction, motility, invasion, glaucoma, asthma, cancer, and cardiovascular mechanisms, although many compounds inhibit both ROCK1 and ROCK2[7].
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Subcellular Localization
Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Golgi apparatus membrane; Peripheral membrane protein; Cell projection, bleb; Cytoplasm, cytoskeleton; Cell membrane; Cell projection, lamellipodium; Cell projection, ruffle
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Expression
Tissue_specificity:Detected in platelets -
Subunit
Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Interacts with SHROOM3 (PubMed:22493320)
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SwissProt ID
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Synonyms
ROCK1; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK
Documentation
References
[1]. Julian L, et al. Rho-associated coiled-coil containing kinases (ROCK): structure, regulation, and functions. Small GTPases. 2014;5:e29846. [Content Brief]
[2]. Riento K, et al. Rocks: multifunctional kinases in cell behaviour. Nat Rev Mol Cell Biol. 2003 Jun;4(6):446-56. [Content Brief]
[3]. Sebbagh M, et al. Caspase-3-mediated cleavage of ROCK I induces MLC phosphorylation and apoptotic membrane blebbing. Nat Cell Biol. 2001 Apr;3(4):346-52. [Content Brief]
[4]. Shimizu Y, et al. ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles. J Cell Biol. 2005 Mar 14;168(6):941-53. [Content Brief]
[5]. Shi J, et al. Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment. Cell Death Dis. 2013 Feb 7;4(2):e483. [Content Brief]
[6]. Mertsch S, et al. Opposing signaling of ROCK1 and ROCK2 determines the switching of substrate specificity and the mode of migration of glioblastoma cells. Mol Neurobiol. 2014 Apr;49(2):900-15. [Content Brief]
[7]. Feng Y, et al. Rho Kinase (ROCK) Inhibitors and Their Therapeutic Potential. J Med Chem. 2016 Mar 24;59(6):2269-300. [Content Brief]
[8]. Gurkar AU, et al. Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189. [Content Brief]
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