ROCK1 - Rho associated coiled-coil containing protein kinase 1 Gene

Also Known as ROCK-I; P160ROCK

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 6093

About ROCK1

Cytogenetic location: 18q11.1 Genomic coordinates (GRCh38): 18:20,946,906-21,111,813 (from NCBI)

This gene has 7 transcripts (splice variants), 212 orthologues and 5 paralogues. Ubiquitous expression in bone marrow (RPKM 22.1), fat (RPKM 16.6) and 25 other tissues.

Summary

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

ROCK1 Products (1)

mRNA	Protein	Name
NM_005406.3	NP_005397.1	rho-associated protein kinase 1

Gene Ontology

Molecular Function
Biological Process

Molecular Function GO Annotation	Evidence	References	Source
enables Rho-dependent protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	18573880	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	10652353	GOA
enables protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	10652353	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in Rho protein signal transduction	IGI IGI: Inferred from genetic interaction	27160703	GOA
involved in canonical NF-kappaB signal transduction	IMP IMP: Inferred from mutant phenotype	25816133	GOA
involved in cortical actin cytoskeleton organization	IGI IGI: Inferred from genetic interaction	25816133	GOA
involved in cortical actin cytoskeleton organization	IMP IMP: Inferred from mutant phenotype	25816133	GOA
involved in leukocyte cell-cell adhesion	IDA IDA: Inferred from direct assay	12082081	GOA
involved in leukocyte migration	IDA IDA: Inferred from direct assay	12082081	GOA
involved in leukocyte tethering or rolling	IDA IDA: Inferred from direct assay	12082081	GOA
involved in membrane to membrane docking	IDA IDA: Inferred from direct assay	12082081	GOA
involved in negative regulation of amyloid precursor protein catabolic process	IMP IMP: Inferred from mutant phenotype	24305806	GOA
involved in negative regulation of amyloid-beta formation	IMP IMP: Inferred from mutant phenotype	24305806	GOA
involved in negative regulation of angiogenesis	IMP IMP: Inferred from mutant phenotype	19181962	GOA
involved in negative regulation of bicellular tight junction assembly	IGI IGI: Inferred from genetic interaction	25816133	GOA
involved in negative regulation of protein binding	IDA IDA: Inferred from direct assay	18573880	GOA
involved in neuron projection arborization	IGI IGI: Inferred from genetic interaction	27160703	GOA
involved in neuron projection development	IGI IGI: Inferred from genetic interaction	27160703	GOA
involved in peptidyl-serine phosphorylation	IDA IDA: Inferred from direct assay	18573880	GOA
involved in positive regulation of MAPK cascade	IMP IMP: Inferred from mutant phenotype	28679962	GOA
involved in positive regulation of amyloid-beta clearance	IGI IGI: Inferred from genetic interaction	27853422	GOA
involved in positive regulation of autophagy	IGI IGI: Inferred from genetic interaction	27853422	GOA
involved in positive regulation of cardiac muscle hypertrophy	IMP IMP: Inferred from mutant phenotype	28679962	GOA
involved in positive regulation of connective tissue replacement	IMP IMP: Inferred from mutant phenotype	28679962	GOA
involved in positive regulation of dephosphorylation	IMP IMP: Inferred from mutant phenotype	27221467	GOA
involved in positive regulation of gene expression	IMP IMP: Inferred from mutant phenotype	27853422	GOA
involved in positive regulation of phosphatase activity	IMP IMP: Inferred from mutant phenotype	27221467	GOA
involved in protein localization to plasma membrane	IGI IGI: Inferred from genetic interaction	25816133	GOA
involved in protein localization to plasma membrane	IMP IMP: Inferred from mutant phenotype	25816133	GOA
involved in protein phosphorylation	IDA IDA: Inferred from direct assay	23093407	GOA
involved in regulation of actin cytoskeleton organization	IDA IDA: Inferred from direct assay	25911094	GOA
involved in regulation of angiotensin-activated signaling pathway	IMP IMP: Inferred from mutant phenotype	28679962	GOA
involved in regulation of cell migration	IDA IDA: Inferred from direct assay	23093407	GOA
involved in regulation of establishment of endothelial barrier	IGI IGI: Inferred from genetic interaction	25816133	GOA
involved in regulation of establishment of endothelial barrier	IMP IMP: Inferred from mutant phenotype	25816133	GOA
involved in regulation of keratinocyte differentiation	IMP IMP: Inferred from mutant phenotype	19997641	GOA
involved in regulation of microtubule cytoskeleton organization	IDA IDA: Inferred from direct assay	23093407	GOA
involved in regulation of neuron differentiation	IGI IGI: Inferred from genetic interaction	27160703	GOA
involved in regulation of synaptic vesicle endocytosis	IGI IGI: Inferred from genetic interaction	27160703	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

ROCK1 Protein Structure

Pkinase

Pkinase_C

HR1

Rho_Binding

0
300
600
900
1200
1354 a.a.

Protein Preferred Names	Protein Names
rho-associated protein kinase 1	p160 ROCK-1 renal carcinoma antigen NY-REN-35

ROCK1 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	ROCK1	Q13464	TSG101	Homo sapiens	Q99816	Y2H	17853893
Intra	ROCK1	Q13464	TSG101	Homo sapiens	Q99816	Anti Tag CoIP	17853893
Intra	ROCK1	Q13464	RHOA	Homo sapiens	P61586	X-Ray Diffraction	14660612
Intra	ROCK1	Q13464	RHOA	Homo sapiens	P61586	Pull Down	21281639
Intra	ROCK1	Q13464	POLR3C	Homo sapiens	Q9BUI4	Y2H Array	25416956
Intra	ROCK1	Q13464	FRMD5	Homo sapiens	Q7Z6J6	Anti Bait CoIP	25448675
Intra	ROCK1	Q13464	LMO2	Homo sapiens	P25791	Validated Y2H	25416956
Intra	ROCK1	Q13464	FAM124A	Homo sapiens	Q86V42	Validated Y2H	25416956
Intra	ROCK1	Q13464	FAM124A	Homo sapiens	Q86V42	Y2H Array	25416956
Intra	ROCK1	Q13464	CFAP206	Homo sapiens	Q8IYR0	Y2H Prey Pooling	25416956
Intra	ROCK1	Q13464	MAGEB4	Homo sapiens	O15481	Y2H Prey Pooling	25416956

Cross: Cross-species interaction Intra: Intraspecies interaction

ROCK1 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P80314	ROCK1 Antibody (YA089)	WB, ICC/IF, IHC-P, IP	Human, Mouse
HY-P86538	ROCK1 Antibody (YA6230)	WB, IHC-P, ICC/IF, IP, ELISA	Human, Mouse, Rat

Related Diseases

Diseases	Alias
Atrial Septal Defect 2	ASD2 Atrial Heart Septal Defect 2 Atrial Septal Defect-2 Asd Ii Septal Defect, Atrial, Type 2
Breast Cancer	Breast Carcinoma Breast Cancer, Familial Malignant Neoplasm Of Breast Male Breast Cancer Breast Cancer, Susceptibility To Breast Cancer, Early-Onset Malignant Tumor Of Breast Carcinoma Of Male Breast Breast Cancer, Invasive Ductal Breast Cancer, Protection Against Breast Cancer, Somatic Breast Cancer, Male Breast Cancer, Lobular, Somatic Breast Tumor Mammary Cancer Mammary Tumor Malignant Neoplasm Of Male Breast Mammary Carcinoma Male Breast Carcinoma Familial Cancer Of Breast Invasive Ductal Breast Carcinoma Breast Cancer Susceptibility Breast Cancer, Male, Susceptibility To Breast Cancer, Early-Onset, Susceptibility To Malignant Tumor Of The Breast Mammary Neoplasm Primary Breast Cancer Neoplasm Of Male Breast Carcinoma Of Breast Breast Cancer In Men Familial Breast Cancer Cancer Of Breast BC Breast Cancer Familial Breast Cancer Familial Male Breast Cancer, Familial Male Breast Male Carcinoma Breast Neoplasms Breast Neoplasms, Male Mammary Tumors Mammary Carcinomas Cancer, Breast Cancer, Breast, Susceptibility Invasive Breast Ductal Carcinoma Breast Neoplasm Susceptibility To Breast Cancer Mammary Neoplasms Animal Mammary Neoplasms Primary Malignant Neoplasm Of Breast Infiltrating Ductal Carcinoma Of Breast Infiltrating Duct Carcinoma Of Unspecified Site Infiltrating Ductular Carcinoma Of Unspecified Site Invasive Breast Carcinoma Of No Special Type Microinvasive Carcinoma Of Breast Carcinoma With Apocrine Differentiation
Lung Cancer	Lung Carcinoma Non-Small Cell Lung Carcinoma Lung Cancer, Protection Against Lung Cancer, Susceptibility To Adenocarcinoma Of Lung, Somatic Nonsmall Cell Lung Cancer Adenocarcinoma Of Lung, Response To Tyrosine Kinase Inhibitor In Lung Neoplasm Carcinoma Of Lung Lung Non-Small Cell Carcinoma Non-Small Cell Lung Cancer Nsclc Lung Neoplasms Malignant Neoplasm Of Lung Alveolar Cell Carcinoma Nonsmall Cell Lung Cancer, Somatic Nonsmall Cell Lung Cancer, Response To Tyrosine Kinase Inhibitor In Nonsmall Cell Lung Cancer, Susceptibility To Lung Cancer, Somatic Lung Cancer, Resistance To Cancer Of Lung Cancer Of Bronchus Cancer Of The Lung Lung Malignancies Lung Malignant Tumors Malignant Lung Tumor Malignant Tumor Of Lung Pulmonary Cancer Pulmonary Carcinoma Pulmonary Neoplasms Respiratory Carcinoma LNCR Adenocarcinoma Of Lung Neoplasm Of Lung Cancer Lung Carcinoma Non-Small Cell Lung Carcinoma, Non-Small-Cell Lung Lung Cancers Lung Carcinomas Cancer, Lung Cancer, Lung, Non-Small Cell Primary Malignant Neoplasm Of Lung Bronchioloalveolar Adenocarcinoma
Hepatocellular Carcinoma	Liver Cancer Primary Liver Cancer HCC Hepatoma Malignant Neoplasm Of Liver Liver Neoplasms Cancer, Hepatocellular Liver Cell Carcinoma Lcc Hepatoblastoma, Somatic Hepatic Cancer Primary Malignant Neoplasm Of Liver Rare Tumor Of Liver And Intrahepatic Biliary Tract Hepatocellular Carcinoma, Somatic Hepatocellular Carcinoma, Childhood Type, Somatic Hepatocellular Cancer, Somatic Ca Liver - Primary Hepatic Neoplasm Malignant Hepato-Biliary Neoplasm Malignant Neoplasm Of Liver, Not Specified As Primary Or Secondary Malignant Neoplasm Of Liver, Primary Malignant Tumor Of Liver Neoplasm Of Liver Non-Resectable Primary Hepatic Malignant Neoplasm Resectable Malignant Neoplasm Of Liver Resectable Malignant Neoplasm Of The Liver Primary Liver Carcinoma Primary Malignant Liver Neoplasm Primary Cancer Of Liver Primary Tumor Of The Liver Rare Tumor Of Liver And Ibt Hepatocellular Cancer Neoplasm Of The Liver Hepatomas Liver Neoplasm Liver Carcinoma Liver And Intrahepatic Biliary Tract Carcinoma Malignant Hepatobiliary Neoplasm Adult Primary Hepatocellular Carcinoma Hepatoblastoma Carcinoma Of Liver Malignant Liver Tumour Malignant Hepatic Tumour
Tetralogy Of Fallot	TOF Fallot Tetralogy Ventricular Septal Defect With Pulmonary Stenosis Or Atresia, Dextraposition Of Aorta, And Hypertrophy Of Right Ventricle Tetrad Of Fallot Fallot Tetrad Fallot Disease Fallot Complex Subpulmonic Stenosis, Ventricular Septal Defect, Overriding Aorta, And Right Ventricular Hypertrophy Interventricular Septal Defect With Dextroposition Of Aorta, Pulmonary Stenosis And Hypertrophy Of Right Ventricle Interventricular Septal Defect, In Tetralogy Of Fallot Ventricular Septal Defect With Obstructed Right Ventricular Outflow Tof - [Tetralogy Of Fallot] Pulmonary Atresia With Ventricular Septal Defect [Fallot Type] Pulmonary Atresia, Ventricular Septal Defect And Mapcas Pulmonary Atresia With Ventricular Septal Defect And Systemic-To-Pulmonary Collateral Arteries [Fallot Type]
Coronary Artery Vasospasm	Coronary Vasospasm Coronary Artery Spasm
Omphalocele	Omphalocoele Congenital Omphalocele Exomphalos Exumbilication

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Approval
Clinical Phase
Pre-clinical Phase

Approval

Bioactive Molecules (11)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-10583	Y-27632 dihydrochloride	Inhibitor	99.98%	Unkown
HY-10341	Fasudil Hydrochloride	Inhibitor	99.97%	Yes
HY-15307	Belumosudil	Inhibitor	99.83%	Yes
HY-10341A	Fasudil	Inhibitor	99.98%	Yes
HY-15685	Ripasudil	Inhibitor	99.95%	Unkown
HY-15307A	Belumosudil mesylate	Inhibitor	99.89%	Yes
HY-13911A	Hydroxyfasudil hydrochloride	Inhibitor	98.88%	Unkown
HY-13911	Hydroxyfasudil	Inhibitor	98.78%	Unkown
HY-10341C	Fasudil dihydrochloride	Inhibitor	99.98%	Yes
HY-10341B	Fasudil hydrochloride semihydrate	Inhibitor	/	Unkown
HY-15685A	Ripasudil free base	Inhibitor	/	Unkown

Clinical Phase

Bioactive Molecules (7)

Cat. No.	Product Name	Effect	Purity	Phase
HY-109191	Sovesudil	Inhibitor	99.91%	Phase 3
HY-109191A	Sovesudil hydrochloride	Inhibitor	99.45%	Phase 3
HY-15687A	SAR407899	Inhibitor	99.67%	Phase 2
HY-15687	SAR407899 hydrochloride	Inhibitor	99.14%	Phase 2
HY-16758	Verosudil	Inhibitor	98.22%	Phase 2
HY-16758A	Verosudil hydrochloride	Inhibitor	/	Phase 2
HY-16071	AT13148	Inhibitor	99.20%	Phase 1

Pre-clinical Phase

Bioactive Molecules (39)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-10071	Y-27632	Inhibitor	99.91%	Unkown
HY-15392	Chroman 1	Inhibitor	98.97%	Unkown
HY-15556	GSK269962A	Inhibitor	99.75%	Unkown
HY-11000	GSK429286A	Inhibitor	98.27%	Unkown
HY-119937	ROCK inhibitor-2	Inhibitor	99.92%	Unkown
HY-15755	RKI-1447	Inhibitor	98.02%	Unkown
HY-13300	SR-3677	Inhibitor	99.23%	Unkown
HY-10319	BAY-549	Inhibitor	99.46%	Unkown
HY-108518	SB-772077B dihydrochloride	Inhibitor	99.33%	Unkown
HY-15392A	Chroman 1 dihydrochloride	Inhibitor	98.13%	Unkown
HY-12437	BDP5290	Inhibitor	98.90%	Unkown
HY-18990	GSK180736A	Inhibitor	98.81%	Unkown
HY-15556A	GSK269962A hydrochloride	Inhibitor	99.73%	Unkown
HY-162596	NRL-1049	Inhibitor	99.96%	Unkown
HY-132302	Hu7691	Inhibitor	99.91%	Unkown
HY-14362	GSK-25	Inhibitor	98.33%	Unkown
HY-149266	THK01	Inhibitor	99.80%	Unkown
HY-110339	RKI-1447 dihydrochloride	Inhibitor	99.94%	Unkown
HY-Q22471	ROCK1-IN-1	Inhibitor	99.14%	Unkown
HY-100270	Rho-Kinase-IN-1	Inhibitor	99.91%	Unkown
HY-125221	DJ4	Inhibitor	99.25%	Unkown
HY-126275	HSD1590	Inhibitor	99.00%	Unkown
HY-14227	LIMK-IN-1	Inhibitor	99.33%	Unkown
HY-122011	PF-4950834	Inhibitor	99.60%	Unkown
HY-178821	ROCK2-IN-12	Inhibitor	98.02%	Unkown
HY-N12378	β-Patchoulene	Inhibitor	96.88%	Unkown
HY-107209	RKI-1313	Inhibitor	98.2%	Unkown
HY-172177	ROCK/HDAC-IN-2	Inhibitor	/	Unkown
HY-132302A	Hu7691 free base	Inhibitor	/	Unkown
HY-13300A	SR-3677 dihydrochloride	Inhibitor	/	Unkown
HY-181582	LASSBio-2382	Inhibitor	/	Unkown
HY-W047432	5-Nitro-1H-indazole-3-carbonitrile	Inhibitor	/	Unkown
HY-178013	ROCK2-IN-11	Inhibitor	/	Unkown
HY-175843	ROCK2-IN-10	Inhibitor	/	Unkown
HY-164809	Desisopropyle-belumosudil	Inhibitor	/	Unkown
HY-181583	LASSBio-2389	Inhibitor	/	Unkown
HY-168650	ROCK/HDAC-IN-1	Inhibitor	/	Unkown
HY-123939	JAK1/3-IN-1	Inhibitor	/	Unkown
HY-N15301	Nocarnickelamide B	Inhibitor	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	ROCK1	MGD	MGI:107927
Macaca mulatta	ROCK1	VGNC	VGNC:81559
Bos taurus	ROCK1	VGNC	VGNC:56371
Canis familiaris	ROCK1	VGNC	VGNC:45688
Felis catus	ROCK1	VGNC	VGNC:97608
Rattus norvegicus	ROCK1	RGD	RGD:620424
Others	ROCK1	NCBI