Nocarnickelamide B TFA
Nocarnickelamide B TFA is a ROCK1/2 inhibitor with IC50 values of 14.9 μM and 21.9 μM, respectively. Nocarnickelamide B TFA inhibits the activation of ROCK-regulated cytoskeletal contraction markers (especially myosin light chain) in human trabecular meshwork cells. Nocarnickelamide B TFA can be used in the research of glaucoma.
For research use only. We do not sell to patients.
- Formula: C29H42N6O9·xC2HF3O2
- Molecular Weight:618.68 (free base)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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ROCK1 14.9 μM (IC50) |
ROCK2 21.9 μM (IC50) |
Nocarnickelamide B (Compound 2) (10-30 μM; 6 h) TFA inhibits the phosphorylation of MYPT1 and MLC2, two cytoskeletal contraction markers regulated by ROCK, in human trabecular meshwork cells, without affecting the protein expression of ROCK1 or ROCK2[1].
Nocarnickelamide B (3-30 μM; 72 h) TFA enhances the permeability of TGF-β2-stimulated human trabecular meshwork cell monolayers in a concentration-dependent manner[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human trabecular meshwork (HTM) cells
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Concentration:10, 30 μM
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Incubation Time:6 h
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Result:Significantly inhibited phosphorylation of MYPT1 (Thr696) and MLC2 (Thr18/Ser19), key markers of cytoskeletal contraction.
Did not alter total ROCK1 or ROCK2 protein expression levels.
Chemical Information
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Molecular Weight 618.68 (free base)
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Formula C29H42N6O9·xC2HF3O2
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SMILES
CC(C)C[C@@H](NC(CNC([C@H]1N(C([C@H](N)CC2=CC=CC=C2)=O)C[C@H](O)C1)=O)=O)C(N3C[C@H](O)C[C@H]3C(NCC(O)=O)=O)=O.OC(C(F)(F)F)=O.[x]
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Sequence
{DPhe}-Hyp-Gly-{DLeu}-Hyp-Gly
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Sequence Shortening
{DPhe}-Hyp-G-{DLeu}-Hyp-G
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Nocarnickelamide B TFA Related Classifications
HY-N15301A Related Classifications
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