Chroman 1 dihydrochloride

Cat. No.: HY-15392A Purity: 98.13%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Chroman 1 dihydrochloride is a highly potent and selective ROCK inhibitor. Chroman 1 dihydrochloride is more potent against ROCK2 (IC₅₀=1 pM) than ROCK1 (IC₅₀=52 pM). Chroman 1 dihydrochloride also has inhibitory activity against MRCK, with an IC₅₀ of 150 nM.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 63 publication(s) in Google Scholar

Other Forms of Chroman 1 dihydrochloride:

Chroman 1 In-stock

63 Publications Citing Use of MCE Chroman 1 dihydrochloride

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: Chroman 1 dihydrochloride purchased from MedChemExpress. Usage Cited in: J Med Virol. 2024 Apr;96(4):e29579. [Abstract]; Chroman 1 (50 nM; 24 h). Survivals of differentiated hiPSCs - anterior foregut endoderm (left) and AOs (right) were improved with CEPT cocktail (Chroman 1, Emricasan, Polyamines and trans-ISRIB) treatment compared to Y27632. Phase contrast images were taken after 24 h of seeding (hiPSC) and 8 days of seeding (AOs).

: Chroman 1 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Protoc. 2023 Jan;18(1):58-80. [Abstract]; Chroman 1 (50 nM; 5 min). Fast-acting CEPT (Chroman 1, Emricasan, Polyamines, trans-ISRIB) is cytoprotective and maintains cell morphology of WA09 dissociated cells, whereas ongoing cell contractions lead to abnormal cells in the presence of Y-27632.

: Chroman 1 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Methods. 2021 May;18(5):528-541. [Abstract]; Improved survival of hESCs (WA09) with Chroman 1 (50 nM; 24 h) treatment.

: Chroman 1 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Methods. 2021 May;18(5):528-541. [Abstract]; Chroman 1 (50 nM; 24 h). hESCs (WA09) cells were dissociated with Accutase and plated on VN in E8 medium. CTG was used to quantify viable cells 24 h post-seeding.

: Chroman 1 dihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Methods. 2021 May;18(5):528-541. [Abstract]; Chroman 1 (50 nM; 24 h). Total caspase-3 expression in hESCs (WA09) in comparison to their lineage-committed precursors after directed differentiation into ectoderm (PAX6), mesoderm (Brachyury), and endoderm (SOX17). Note that caspase-3 is strongly expressed in the pluripotent state and downregulated upon differentiation.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]^[2]

ROCK2

1 pM (IC₅₀)

ROCK1

52 pM (IC₅₀)

MRCK

150 nM (IC₅₀)

In Vitro

Chroman 1 (50 nM, 24 h) inhibits caspase-3/7 activation and reduces apoptosis in human pluripotent stem cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:	hESCs (human pluripotent stem cells) (WA09)
Concentration:	50 nM
Incubation Time:	0-12 h or 24 h
Result:	Reduced the number of apoptotic cells, reduced caspase-3/7 activation.

Western Blot Analysis^[1]

Cell Line:	hESCs (human pluripotent stem cells) (WA09)
Concentration:	50 nM
Incubation Time:	24 h
Result:	Partially inhibited caspase-3 activation.

Molecular Weight

509.43

Formula

C₂₄H₃₀Cl₂N₄O₄

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C([C@@H]1COC2=CC=C(OC)C=C2C1)NC3=CC=C(C4=CNN=C4)C=C3OCCN(C)C.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (196.30 mM; Need ultrasonic)

DMSO : 100 mg/mL (196.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.9630 mL	9.8149 mL	19.6298 mL
5 mM	0.3926 mL	1.9630 mL	3.9260 mL
10 mM	0.1963 mL	0.9815 mL	1.9630 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (1.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (1.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (1.96 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 98.13%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (249 KB) HNMR (358 KB) LCMS (105 KB)

Handling Instructions (2659 KB)

References

[1]. Yu Chen, et al. A Versatile Polypharmacology Platform Promotes Cytoprotection and Viability of Human Pluripotent and Differentiated Cells. bioRxiv 815761.

[2]. Yen Ting Chen, et al. Asymmetric synthesis of potent chroman-based Rho kinase (ROCK-II) inhibitors. Med.Chem.Commun., 2011, 2, 73-75.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.9630 mL	9.8149 mL	19.6298 mL	49.0745 mL
	5 mM	0.3926 mL	1.9630 mL	3.9260 mL	9.8149 mL
	10 mM	0.1963 mL	0.9815 mL	1.9630 mL	4.9074 mL
	15 mM	0.1309 mL	0.6543 mL	1.3087 mL	3.2716 mL
	20 mM	0.0981 mL	0.4907 mL	0.9815 mL	2.4537 mL
	25 mM	0.0785 mL	0.3926 mL	0.7852 mL	1.9630 mL
	30 mM	0.0654 mL	0.3272 mL	0.6543 mL	1.6358 mL
	40 mM	0.0491 mL	0.2454 mL	0.4907 mL	1.2269 mL
	50 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9815 mL
	60 mM	0.0327 mL	0.1636 mL	0.3272 mL	0.8179 mL
	80 mM	0.0245 mL	0.1227 mL	0.2454 mL	0.6134 mL
	100 mM	0.0196 mL	0.0981 mL	0.1963 mL	0.4907 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.