Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia

  • STAR Protoc. 2022 Dec 16;3(4):101847. doi: 10.1016/j.xpro.2022.101847.
Mengmeng Jin  1 ,  Ziyuan Ma  2 ,  Peng Jiang  3
Affiliations
  • 1. Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: [email protected].
  • 2. Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
  • 3. Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: [email protected].
PMID: 36595906 DOI: 10.1016/j.xpro.2022.101847
Abstract

Here, we provide a step-by-step protocol for generating human induced pluripotent stem cell (hiPSC)-based microglial mouse brain chimeras. In addition, we detail steps for intracerebral injection of pathological tau and magnetic Cell Isolation of human microglia from chimeric mouse brains for single-cell RNA Sequencing. Human microglia developed in chimeric mouse brains recapitulate the pathophysiology of microglia in human brain tissue, offering unprecedented opportunities to study human microglial senescence in vivo. For complete details on the use and execution of this protocol, please refer to (Jin et al., 2022b).

Keywords
Cell isolation; Model Organisms; Neuroscience; Single Cell; Stem Cells.
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