1. Cell Cycle/DNA Damage
    TGF-beta/Smad
    Stem Cell/Wnt
  2. ROCK
  3. Chroman 1

Chroman 1 

Cat. No.: HY-15392 Purity: 99.76%
Handling Instructions

Chroman 1 is a highly potent ROCK2 inhibitor, with an IC50 of 1 nM.

For research use only. We do not sell to patients.

Chroman 1 Chemical Structure

Chroman 1 Chemical Structure

CAS No. : 1273579-40-0

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 377 In-stock
Estimated Time of Arrival: December 31
5 mg USD 343 In-stock
Estimated Time of Arrival: December 31
10 mg USD 678 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Description

Chroman 1 is a highly potent ROCK2 inhibitor, with an IC50 of 1 nM.

IC50 & Target[1]

ROCK2

1 nM (IC50)

MRCK

150 nM (IC50)

In Vitro

Chroman 1 is a highly potent ROCK2 inhibitor, with an IC50 of < 1 nM. Chroman 1 also shows inhibitory activities against MRCK, with an IC50 of 150 nM, but has no effect on PKA (IC50, > 20000 nM) or AKT1 (IC50, > 20000 nM)[1].

Molecular Weight

436.50

Formula

C₂₄H₂₈N₄O₄

CAS No.

1273579-40-0

SMILES

O=C([[email protected]@H]1COC2=CC=C(OC)C=C2C1)NC3=CC=C(C4=CNN=C4)C=C3OCCN(C)C

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (114.55 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2910 mL 11.4548 mL 22.9095 mL
5 mM 0.4582 mL 2.2910 mL 4.5819 mL
10 mM 0.2291 mL 1.1455 mL 2.2910 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 3.25 mg/mL (7.45 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

Assays are performed using the HTRF KinEASE STK S2 kit. A 5 μL mixture of 1 μM STK2 substrate and 20 μM ATP in STK-buffer is added to the wells. Inhibitor (including Chroman 1, 20 nL) at various concentrations is then dispensed. Reaction is started by addition of 0.5 nM ROCK-II in STK-buffer (5 μL). After 4 h at room temperture (rt), the reaction is stopped by addition of 10 μL mixture of 1× STK antibody-Cryptate in detection buffer and 62.5 nM Sa-XL in detection buffer. After 1 h at rt, the plates are read on the Viewlux in HTRF mode[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.76%

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Chroman 1
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